Assessment of two different matrices for the early detection and isolation of thermophilic Campylobacter in broiler farms.

Assessment of two different matrices for the early detection and isolation of thermophilic Campylobacter in broiler farms.
Avian Pathol. 2015 Jan 15;:1-12
Authors: Urdaneta S, Dolz R, Cerdà-Cuéllar M
Abstract
In order to assess the optimal method for the early detection and isolation of thermophilic Campylobacter in broilers at farm level, two types of samples were compared: caecal contents obtained by necropsy and cloacal swabs transported in charcoal Amies medium. The study was conducted in five batches of broilers from five different farms, where weekly samples (caecal contents and cloacal swabs) from 30 birds were obtained. Samples were plated onto selective agar (mCCDA) for Campylobacter isolation. Four out of five batches were positive for Campylobacter. No marked differences in sensitivity of both sample types were observed. However, a higher percentage of positive birds were detected when cloacal swabs were used. The results show that cloacal swab samples are adequate, and in some cases even better, than caecal samples for the early detection of Campylobacter in broiler flocks at farm. Also, this sample avoids sacrificing birds to test Campylobacter, which not only allows saving time in sample collection, transportation and processing at the laboratory, but also improves bird welfare and cost of sampling.
PMID: 25588419 [PubMed - as supplied by publisher]

Source: Avian Pathology - January 15, 2015 Category: Pathology Authors: Urdaneta S, Dolz R, Cerdà-Cuéllar M Tags: Avian Pathol Source Type: research