A Revised Adaptation of the Smart-Seq2 Protocol for Single-Nematode RNA-Seq.

A Revised Adaptation of the Smart-Seq2 Protocol for Single-Nematode RNA-Seq. Methods Mol Biol. 2021;2170:79-99 Authors: Chang D, Serra L, Lu D, Mortazavi A, Dillman A Abstract The advancement of transcriptomic studies in plant parasitic nematodes will greatly benefit from the development of single-nematode RNA-seq methods. Since many plant parasitic nematodes are obligate parasites, it is often difficult to efficiently obtain sufficient amounts of nematodes for transcriptomic studies. Here we have adapted SMART-Seq2 for single-nematode RNA-seq requiring only an individual nematode for a sample replicate. This protocol provides a detailed step-by-step procedure of the RNA-seq workflow starting from lysis of the nematode to quantification of transcripts using a user-friendly online platform. PMID: 32797452 [PubMed - in process]
Source: Mol Biol Cell - Category: Molecular Biology Authors: Tags: Methods Mol Biol Source Type: research