Photo-Biohydrogen Production by Photosensitization with Biologically Precipitated Cadmium Sulfide in Hydrogen-Forming Recombinant Escherichia coli.

In this study, we aimed to develop a new photocatalytic hydrogen production system, based on Escherichia coli whole-cell genetically engineered for the highly active hydrogen formation as a biocatalysis part. The photocatalysis part was obtained by bacterial precipitation of cadmium sulfide (CdS), which is a visible light responsive semiconductor. The recombinant E. coli cells were sequentially subjected to CdS precipitation and heterologous [FeFe]-hydrogenase synthesis, to yield a CdS@ E. coli hybrid capable of light energy conversion and hydrogen formation in a single cell. The CdS@ E. coli hybrid achieved photocatalytic hydrogen production with a sacrificial electron donor, demonstrating the feasibility of our system and expanding the current knowledge of photosensitization using a whole-cell biocatalyst with a bacterially precipitated semiconductor. PMID: 32697401 [PubMed - as supplied by publisher]
Source: Chembiochem - Category: Biochemistry Authors: Tags: Chembiochem Source Type: research