Genes, Vol. 11, Pages 789: Transcriptional Profiling of Normal, Stenotic, and Regurgitant Human Aortic Valves
Genes, Vol. 11, Pages 789: Transcriptional Profiling of Normal, Stenotic, and Regurgitant Human Aortic Valves
Genes doi: 10.3390/genes11070789
Authors:
Christina L. Greene
Kevin J. Jaatinen
Hanjay Wang
Tiffany K. Koyano
Mary S. Bilbao
Y. Joseph Woo
The genetic mechanisms underlying aortic stenosis (AS) and aortic insufficiency (AI) disease progression remain unclear. We hypothesized that normal aortic valves and those with AS or AI all exhibit unique transcriptional profiles. Normal control (NC) aortic valves were collected from non-matched donor hearts that were otherwise acceptable for transplantation (n = 5). Valves with AS or AI (n = 5, each) were collected from patients undergoing surgical aortic valve replacement. High-throughput sequencing of total RNA revealed 6438 differentially expressed genes (DEGs) for AS vs. NC, 4994 DEGs for AI vs. NC, and 2771 DEGs for AS vs. AI. Among 21 DEGs of interest, APCDD1L, CDH6, COL10A1, HBB, IBSP, KRT14, PLEKHS1, PRSS35, and TDO2 were upregulated in both AS and AI compared to NC, whereas ALDH1L1, EPHB1, GPX3, HIF3A, and KCNT1 were downregulated in both AS and AI (p < 0.05). COL11A1, H19, HIF1A, KCNJ6, PRND, and SPP1 were upregulated only in AS, and NPY was downregulated only in AS (p < 0.05). The functional network for AS clustered around ion regulation, immune regulation, and lipid homeostasis, and that for AI clustered around ERK1/2 regulation. Overall, we report transcriptional profiling data...
Source: Genes - Category: Genetics & Stem Cells Authors: Christina L. Greene Kevin J. Jaatinen Hanjay Wang Tiffany K. Koyano Mary S. Bilbao Y. Joseph Woo Tags: Article Source Type: research
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