Enriched terpenes fractions of Euphorbia umbellata latex promote apoptosis in leukemic cells.

Enriched terpenes fractions of Euphorbia umbellata latex promote apoptosis in leukemic cells. Chem Biodivers. 2020 Jul 09;: Authors: Cruz LS, Kanunfre CC, de Andrade EA, de Oliveira AA, Cruz LS, de Faria Moss M, Sassaki GL, Alencar Menezes LR, Wang M, Khan IA, Beltrame FL Abstract The current study was carried out by a bioguided-fractionation of a hexane extract of Euphorbia umbellata latex against leukemic cells. Samples were analyzed by NMR, GC-MS, triterpenes quantification, and MTT reduction assay. Morphological, cell cycle, mitochondrial membrane potential and caspases 3/7 analyses were performed for the dichloromethane and ethanol fractions, and selectivity index for the dichloromethane fraction. NMR analysis presented characteristic signals of terpenes and steroids, data were confirmed by the quantification of triterpenes and GC-MS analysis. MTT reduction assay demonstrated that HL-60 was the most sensitive cell lineage against dichloromethane and ethanol fractions. Compounds of these matrices caused morphological changes compatible with apoptosis induction, altered cell cycle, increment of depolarized population cells and activation of caspases 3/7. Selectivity indices was higher than 22.44. Bioguided-fractionation study showed that samples of the latex of E. umbellata raised the activity of the phytocomplex against leukemic cells, and the cytotoxicity can be associated with an apoptosis pathway.The current study was carried ...
Source: Chemistry and Biodiversity - Category: Biochemistry Authors: Tags: Chem Biodivers Source Type: research