GSE154069 The Role of Satb1 in Shaping the Repertoire of T Cell Receptor Genes

Contributors : Delong Feng ; Zhaoqiang LiSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusTo explore the role of Satb1 in rearrangement and repertoire of TCR genes, we used Satb1contional knockout micwhich the Satb1 gene was deleted in long term hematopoietic stem cells. We applied 5 ʹRACE (rapid amplification of cDNA ends) PCR technique to generate deep sequencing libraries of Tcrd, Tcrg, Tcrb, and Tcra cDNA by using a single set of primers for each TCR gene. We obtained millions of TCR sequences and unraveled the complexity of T-cell diversity. Our experimental evidence demon strates that Satb1 has substantial influences on the TCR repertoire.
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research