Enhanced Collection of Phenotypic and Engrafting Human Cord Blood Hematopoietic Stem Cells at 4 °C

AbstractHematopoietic stem cell (HSC) numbers collected in cord blood (CB) at the birth of a baby is a limiting factor for efficacious use of CB in hematopoietic cell transplantation (HCT). We now demonstrate that collecting and processing of human CB at 4 °C within minutes of the baby's birth results in significantly enhanced numbers of rigorously defined phenotypic HSC and self‐renewing NSG immune‐deficient mouse engrafting and SCID‐repopulating cells. This was associated with decreased numbers of hematopoietic progenitor cells (HPC), as note d previously for hypoxia collected/processed cells blocking ambient air induced differentiation of HSC to HPC. We have thus defined a simple, cost‐effective, means to collect increased numbers of CB HSC, of potential use for clinical CB HCT.© AlphaMed Press 2020Significance StatementNumbers of collected cord blood hematopoietic stem cells at the birth of a baby is a limiting factor in use of cord blood for hematopoietic cell transplantation. We now demonstrate, by collecting and processing cord blood at 4 °C, that we can attain significantly enhanced numbers of cord blood hematopoietic stem cells for potential use in clinical cord blood hematopoietic cell transplantation, in a simple and cost‐effective manner.
Source: Stem Cells - Category: Stem Cells Authors: Tags: Tissue ‐Specific Stem Cells Source Type: research