Molecules, Vol. 25, Pages 2981: Biological Activity of Porcine Gastric Mucin on Stress Resistance and Immunomodulation

Molecules, Vol. 25, Pages 2981: Biological Activity of Porcine Gastric Mucin on Stress Resistance and Immunomodulation Molecules doi: 10.3390/molecules25132981 Authors: Thiloma D. Liyanage Pasan S. Dahanayake Shan L. Edirisinghe Chamilani Nikapitiya Gang-Joon Heo Mahanama De Zoysa Ilson Whang Purified porcine gastric mucin (PGM) is an alternative biomaterial to native mucin which displays multifunctional properties for exploring a wide range of biomedical applications. The present study evaluated the in vitro (RAW 264.7 macrophage cells) and in vivo (zebrafish embryos and larvae) bioactivities of PGM. The median lethal concentration (LC50) of PGM was 197.9 µg/mL for embryos, while it was non-toxic to RAW 264.7 cells, even at 500 µg/mL. Following PGM exposure (100 µg/mL), a higher embryo hatching rate (59.9%) was observed at 48 h post fertilization, compared to the control (30.6%). Protective effects of PGM from pathogenic Aeromonas hydrophila were demonstrated by high larvae survival rates of 85.0% and 94.0% at 50 and 100 μg/mL of PGM exposure, respectively. Heat tolerance effect of PGM (50 and 100 µg/mL) on larvae (40 °C for 48 h) was confirmed by 75% and 100% of survival rates, respectively. Additionally, PGM reduced the A. hydrophila–induced reactive oxygen species (ROS) generation in larvae. The qRT-PCR results in PGM exposed larvae exhibited induction of immun...
Source: Molecules - Category: Chemistry Authors: Tags: Article Source Type: research