Chronic Imaging of Mouse Brain: From Optical Systems to Functional Ultrasound.

Chronic Imaging of Mouse Brain: From Optical Systems to Functional Ultrasound. Curr Protoc Neurosci. 2020 Sep;93(1):e98 Authors: Kılıç K, Tang J, Erdener ŞE, Sunil S, Giblin JT, Lee BS, Postnov DD, Chen A, Boas DA Abstract Utilization of functional ultrasound (fUS) in cerebral vascular imaging is gaining popularity among neuroscientists. In this article, we describe a chronic surgical preparation method that allows longitudinal studies and therefore is applicable to a wide range of studies, especially on aging, stroke, and neurodegenerative diseases. This method can also be used with awake mice; hence, the deleterious effects of anesthesia on neurovascular responses can be avoided. In addition to fUS imaging, this surgical preparation allows researchers to take advantage of common optical imaging methods to acquire complementary datasets to help increase the technical rigor of studies. © 2020 Wiley Periodicals LLC. Basic Protocol 1: Surgical preparation of mouse chronic cranial windows using polymethylpentene Basic Protocol 2: Imaging of mice with chronic cranial windows. PMID: 32584495 [PubMed - in process]
Source: Current Protocols in Neuroscience - Category: Neuroscience Authors: Tags: Curr Protoc Neurosci Source Type: research