Net release and uptake of xenometabolites across intestinal, hepatic, muscle, and renal tissue beds in healthy conscious pigs.

Net release and uptake of xenometabolites across intestinal, hepatic, muscle, and renal tissue beds in healthy conscious pigs. Am J Physiol Gastrointest Liver Physiol. 2020 Jun 15;: Authors: Mercer KE, Ten Have GAM, Pack L, Lan R, Deutz NEP, Adams SH, Piccolo BD Abstract Xenometabolites from microbial and plant sources are thought to confer beneficial, as well as deleterious, effects on host physiology. Studies determining absorption and tissue uptake of xenometabolites are limited. We utilized a conscious catheterized pig model to evaluate inter-organ flux of annotated known and suspected xenometabolites, derivatives, and bile acids. Female pigs (n=12; 2-3 months old; 25.6 ± 2.2 kg) had surgically-implanted catheters across portal-drained viscera (PDV), splanchnic area (SPL), liver, kidney, and hindquarter muscle. Overnight fasted arterial and venous plasma was collected simultaneously in a conscious state and stored at -80°C. Thawed samples were analyzed by liquid chromatography-mass spectrometry. Plasma flow was determined with para-aminohippuric acid dilution technology and used to calculate net organ balance for each metabolite. Significant organ uptake or release was determined if net balance differed from zero. A total of 48 metabolites were identified in plasma, and 31 of these had at least one tissue with a significant net release or uptake. All bile acids, indole-3-acetic acid, indole-3-arylic acid, and hydrocinnamic acid...
Source: Am J Physiol Gastroi... - Category: Gastroenterology Authors: Tags: Am J Physiol Gastrointest Liver Physiol Source Type: research