Cancers, Vol. 12, Pages 1468: Potential Onco-Suppressive Role of miR122 and miR144 in Uveal Melanoma through ADAM10 and C-Met Inhibition

Cancers, Vol. 12, Pages 1468: Potential Onco-Suppressive Role of miR122 and miR144 in Uveal Melanoma through ADAM10 and C-Met Inhibition Cancers doi: 10.3390/cancers12061468 Authors: Adriana Amaro Michela Croce Silvano Ferrini Gaia Barisione Marina Gualco Patrizia Perri Ulrich Pfeffer Martine J. Jager Sarah E. Coupland Carlo Mosci Gilberto Filaci Marina Fabbi Paola Queirolo Rosaria Gangemi Uveal melanoma (UM) is a rare tumor of the eye that leads to deadly metastases in about half of the patients. ADAM10 correlates with c-Met expression in UM and high levels of both molecules are related to the development of metastases. MiR122 and miR144 modulate ADAM10 and c-Met expression in different settings. We hypothesized a potential onco-suppressive role for miR122 and miR144 through modulation of ADAM10 and c-Met in UM. We analyzed the UM Cancer Genome Atlas data portal (TCGA) dataset, two other cohorts of primary tumors and five human UM cell lines for miR122 and miR144 expression by miR microarray, RT-qPCR, Western blotting, miR transfection and luciferase reporter assay. Our results indicate that miR122 and miR144 are expressed at low levels in the UM cell lines and in the TCGA UM dataset and were down-modulated in a cohort of seven UM samples, compared to normal choroid. Both miR122 and miR144 directly targeted ADAM10 and c-Met. Overexpression of miR122 and miR144 led to reduced expression of ADAM10 and c-Met in the UM cell lines and impaired ce...
Source: Cancers - Category: Cancer & Oncology Authors: Tags: Article Source Type: research