Renal ischemia alters expression of mitochondria-related genes and impairs mitochondrial structure and function in swine scattered tubular-like cells.

Renal ischemia alters expression of mitochondria-related genes and impairs mitochondrial structure and function in swine scattered tubular-like cells. Am J Physiol Renal Physiol. 2020 May 28;: Authors: Farahani RA, Zhu XY, Tang H, Jordan KL, Lerman LO, Eirin A Abstract Scattered tubular-like cells (STCs) are dedifferentiated surviving tubular epithelial cells that repair neighboring injured cells. Experimental renal artery stenosis (RAS) impairs STC reparative potency by inducing mitochondrial injury, but the exact mechanisms of mitochondrial damage remains unknown. We hypothesized that RAS alters expression of mitochondria-related genes, contributing to mitochondrial structural damage and dysfunction in swine STCs. CD24+/CD133+ STCs were isolated from pig kidneys after 10 weeks of RAS or sham (n=3 each). mRNA sequencing was performed and nuclear-DNA (nDNA) encoded mitochondrial genes and mitochondrial-DNA (mtDNA) encoded genes were identified. Mitochondrial structure, ATP generation, biogenesis, and expression of mitochondria-associated microRNAs (mitomiRs) were also assessed. There were 96 nDNA-encoded mitochondrial genes upregulated and 12 mtDNA-encoded genes downregulated in RAS-STCs versus Normal-STCs. Functional analysis revealed that nDNA-encoded and mtDNA-encoded differentially-expressed genes were primarily implicated in mitochondrial respiration and ATP synthesis. Mitochondria from RAS-STCs were swollen and showed cristae r...
Source: American Journal of Physiology. Renal Physiology - Category: Physiology Authors: Tags: Am J Physiol Renal Physiol Source Type: research