Physical and biological properties of blend ‐electrospun polycaprolactone/chitosan‐based wound dressings loaded with N‐decyl‐N, N‐dimethyl‐1‐decanaminium chloride: An in vitro and in vivo study

AbstractDual ‐pump electrospinning of antibacterial N‐decyl‐N, N‐dimethyl‐1‐decanaminium‐chloride (DDAC)‐loaded polycaprolactone (PCL) nanofibers, and chitosan (CS)/polyethylene‐oxide (PEO)‐based wound dressings with hydrophilic and hydrophobic properties to eliminate and absorb pathogenic ba cteria from wound surface besides antibacterial action and to support wound healing and accelerate its process. Physicochemical properties of the prepared nanofibrous mat as well as antibacterial, cytotoxicity, and cell compatibility were studied. The full‐thickness excisional wound healing proper ties up to 3 weeks using hematoxylin and eosin and Masson‐trichrome staining were investigated. Addition of DDAC to CS/PEO‐PCL mats decreased the diameter of the nanofibers, which is a crucial property for wound healing as large surface area per volume ratio of nanofibers, in addition to prope r cell adhesion, increases loading of DDAC in mats and leads to increased cell viability and eliminating Gram‐positive bacteria at in vitro studies. In vivo studies showed DDAC‐loaded CS/PEO‐PCL mats increased epithelialization and angiogenesis and decreased the inflammation according to histo logical results. We demonstrated that hydrophobic PCL/DDAC mats, besides antibacterial properties of DDAC, absorbed and eliminated the hydrophobic pathological microorganisms, whereas the hydrophilic nanofibers consisted of CS/PEO, increased the cell adhesion and proliferation due t...
Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - Category: Materials Science Authors: Tags: ORIGINAL RESEARCH REPORT Source Type: research