Effects of photobiomodulation at different wavelengths on orthodontically induced root resorption in orthodontic retention period: a micro-CT and RT-PCR study

AbstractThe aim of this study was to evaluate and compare the reparative and inhibitory effects of single wavelength photobiomodulation (SW-PBM) and of cumulative increased wavelength photobiomodulation (CW-PBM) on orthodontically induced inflammatory root resorption (OIIRR). Thirty-three Wistar albino rats were divided into five groups: untreated group (negative control), only relapse group (positive control-1), only retention group (positive control-2), SW-PBM group (650  nm, 100 mW/cm2), and CW-PBM group (532 –650–940 nm, 100 mW/cm2). Orthodontic tooth movement was induced experimentally in rats for 10  days with an applied force of 50 cN; retention and therapeutic approaches were performed concurrently. At the end of the experiment, maxillary quadrants were prepared for micro-CT analysis and real-time polymerase chain reaction (RT-PCR) analysis. After the Shapiro-Wilk normality test, Kruskal-Wal lis test followed post hoc Bonferroni test and paired samplest test was used for statistical evaluation of the data. Resorption lacunae volume (p <  0.001), number of resorption lacunae (p <  0.05), and percentage of the resorption (PR) lacunae (p <  0.001) decreased with PBM applications when compared with the positive control groups, and the mean PR was similar in the negative control group when compared with SW-PBM group. Receptor activator of nuclear factor kappa B ligand (RANKL) levels of the PBM groups were lower (p <  0.05) than those of...
Source: Lasers in Medical Science - Category: Laser Surgery Source Type: research