Kisspeptin induces LH release and ovulation in an induced ovulator.

Kisspeptin induces LH release and ovulation in an induced ovulator. Biol Reprod. 2020 Apr 20;: Authors: Carrasco RA, Leonardi CE, Hutt K, Singh J, Adams GP Abstract Kisspeptin has been implicated in the ovulatory process of several species of spontaneous ovulators, but in only one induced ovulator. In contrast, NGF in semen is the principal trigger of ovulation in other species of induced ovulators-camelids. We tested the hypotheses that kisspeptin induces LH secretion in llamas through a hypothalamic mechanism, and kisspeptin neurons are the target of NGF in its ovulation-inducing pathway. In Experiment 1, llamas were given either NGF, kisspeptin or saline intravenously, and luteinizing hormone (LH) secretion and ovulation were compared among groups. All llamas treated with NGF (5/5) or kisspeptin (5/5) had an elevation of LH blood concentrations after treatment and ovulated whereas none of the saline group did (0/5). In Experiment 2, llamas were either pre-treated with a gonadotropin-releasing hormone (GnRH) receptor antagonist or saline, and treated two hours later with kisspeptin. Llamas pre-treated with saline had elevated plasma LH concentrations and ovulated (6/6) whereas llamas pre-treated with cetrorelix did not (0/6). In Experiment 3, we evaluated the hypothalamic kisspeptin-GnRH neuronal network by immunohistochemistry. Kisspeptin neurons were detected in the arcuate nucleus, the preoptic area and the anterior hypothalamus...
Source: Biology of Reproduction - Category: Reproduction Medicine Authors: Tags: Biol Reprod Source Type: research