Comparative transcriptome and phenotype analysis revealed the role and mechanism of ompR in the virulence of fish pathogenic Aeromonas hydrophila

In this study, theompR ofA.  hydrophila B11 strain was first silenced by RNAi technology. The role ofompR in the pathogenicity ofA.  hydrophila B11 was investigated by analyzing both the bacterial comparative transcriptome and phenotype. The qRT ‐PCR results showed that the expression ofompR in theompR‐RNAi strain decreased by 97% compared with the wild‐type strain. The virulence test showed that after inhibition of theompR expression, the LD50 ofA.  hydrophila B11 decreased by an order of magnitude, suggesting thatompR is involved in the regulation of bacterial virulence. Comparative transcriptome analysis showed that the expression ofompR can directly regulate the expression of several important virulence ‐related genes, such as the bacterial type II secretion system; moreover,ompR expression also regulates the expression of multiple genes related to bacterial chemotaxis, motility, adhesion, and biofilm formation. Further studies on the phenotype ofA.  hydrophila B11 andompR‐RNAi also confirmed that the downregulation ofompR expression can decrease bacterial chemotaxis, adhesion, and biofilm formation.
Source: MicrobiologyOpen - Category: Microbiology Authors: Tags: ORIGINAL ARTICLE Source Type: research