Effects of anastrozole on Ki-67 antigen expression in the vaginal epithelium of female rats in persistent estrus

OBJECTIVES: Aromatase inhibitors are the first-choice drugs for the treatment of hormone sensitive breast cancer. However, in addition to the scarcity of studies, there are controversies about their effects on vaginal epithelial cell proliferation in rats, especially those in persistent estrus. METHODS: To investigate vaginal epithelial cell proliferation by Ki-67 antigen expression, persistent estrus was induced in 42 randomly selected rats. These rats were randomly divided into 2 groups: group I (control, n=21), which received 0.1 mL of propylene glycol (vehicle) daily, and group II (experimental, n=21), which received 0.5 mg/kg or 0.125 mg/day of anastrozole diluted with 0.1 mL of propylene glycol. RESULTS: Light microscopy showed a higher concentration of cells with brown Ki-67 stained nuclei in the control compared to the experimental group. The mean percentage of Ki-67 stained nuclei per 500 cells in the vaginal epithelium was 68.64 ±2.64 and 30.46±2.00 [mean±standard error of the mean (SEM)] in the control and experimental groups, respectively (p
Source: Clinics - Category: General Medicine Source Type: research

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Source: Al Sears, MD Natural Remedies - Category: Complementary Medicine Authors: Tags: Health Source Type: news
Long non-coding RNA H19 (lncRNA H19) has been implicated in tumorigenesis and metastasis of breast cancer through regulating epithelial to mesenchymal transition (EMT); however, the underlying mechanisms remai...
Source: Cancer Cell International - Category: Cancer & Oncology Authors: Tags: Primary research Source Type: research
This study highlights the importance of global AS regulation during EMT in cancer progression and paves the way for further investigation into RNA regulation in EMT and metastasis. PMID: 32467311 [PubMed - as supplied by publisher]
Source: RNA - Category: Genetics & Stem Cells Authors: Tags: RNA Source Type: research
The RNA-binding protein Musashi-2 (MSI2) has been implicated in the tumorigenesis and tumor progression of some human cancers. MSI2 has also been reported to suppress tumor epithelial-to-mesenchymal transition...
Source: Journal of Experimental and Clinical Cancer Research - Category: Cancer & Oncology Authors: Tags: Research Source Type: research
Contributors : Pierre Bourdely ; Kristine Vaivode ; Giorgio Anselmi ; Rodrigo N Ramos ; Yoann Missolo-Kousou ; Sofia Hidalgo ; Jimena Tosselo ; Nicolas Nunez ; Wilfrid Richer ; Anne Vincent-Salomon ; Alka Saxena ; Kristie Wood ; Alvaro Lladser ; Eliane Piaggio ; Julie Helft ; Pierre GuermonprezSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensDendritic cells (DC) are antigen presenting cells controlling T cell activation. In human, the diversity, ontogeny and functional capabilities of DC subsets are not fully understood. Here, we identified circulating CD88-CD1c+CD163+ DC (termed as DC...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research
Contributors : Pierre Bourdely ; Kristine Vaivode ; Giorgio Anselmi ; Rodrigo N Ramos ; Yoann Missolo-Kousou ; Sofia Hidalgo ; Jimena Tosselo ; Nicolas Nunez ; Wilfrid Richer ; Anne Vincent-Salomon ; Alka Saxena ; Kristie Wood ; Alvaro Lladser ; Eliane Piaggio ; Julie Helft ; Pierre GuermonprezSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensDendritic cells (DC) are antigen presenting cells controlling T cell activation. In human, the diversity, ontogeny and functional capabilities of DC subsets are not fully understood. Here, we identified circulating CD88-CD1c+CD163+ DC (termed as DC...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research
Contributors : Pierre Bourdely ; Kristine Vaivode ; Giorgio Anselmi ; Rodrigo N Ramos ; Yoann Missolo-Kousou ; Sofia Hidalgo ; Jimena Tosselo ; Nicolas Nunez ; Wilfrid Richer ; Anne Vincent-Salomon ; Alka Saxena ; Kristie Wood ; Alvaro Lladser ; Eliane Piaggio ; Julie Helft ; Pierre GuermonprezSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensDendritic cells (DC) are antigen presenting cells controlling T cell activation. In human, the diversity, ontogeny and functional capabilities of DC subsets are not fully understood. Here, we identified circulating CD88-CD1c+CD163+ DC (termed as DC...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research
Contributors : Pierre Bourdely ; Kristine Vaivode ; Giorgio Anselmi ; Rodrigo N Ramos ; Yoann Missolo-Kousou ; Sofia Hidalgo ; Jimena Tosselo ; Nicolas Nunez ; Wilfrid Richer ; Anne Vincent-Salomon ; Alka Saxena ; Kristie Wood ; Alvaro Lladser ; Eliane Piaggio ; Julie Helft ; Pierre GuermonprezSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensDendritic cells (DC) are antigen presenting cells controlling T cell activation. In human, the diversity, ontogeny and functional capabilities of DC subsets are not fully understood. Here, we identified circulating CD88-CD1c+CD163+ DC (termed as DC...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research
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Source: Breast Cancer - Category: Cancer & Oncology Authors: Tags: Breast Cancer Source Type: research
This article provides a set of protocols for generating uniform spheroids with a thin sheet of basement membrane for studying the initial invasion of mammary epithelial cells into a surrounding collagen-rich extracellular matrix. Details are provided for generating 3D spheroids with a basement membrane, polymerizing collagen I, embedding the spheroids in the 3D collagen gel, and immunostaining the spheroids for invasion studies. Published 2020. U.S. Government. Basic Protocol 1: Growth of uniformly sized tumor spheroids with an encapsulating basement membrane Basic Protocol 2: Polymerization and embedding of tumor spheroid...
Source: Current Protocols in Cell Biology - Category: Cytology Authors: Tags: Curr Protoc Cell Biol Source Type: research
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