Tandem repeat analysis for typing of Pseudomonas aeruginosa carrying resistance genes in southern Iran

Pseudomonas aeruginosa is known to be one of the most important causes of nosocomial infections, with high antimicrobial resistance. Thus, the current study was conducted to evaluate usefulness of multiple-locus variable number of tandem repeat (VNTR) analysis (MLVA) plan for epidemiological studies and clustering of P. aeruginosa isolated in different wards of general hospital in Bandar Abbas, south of Iran, as well as assessment of presence of integrons and resistance genes in these strains. A total of 72 clinical isolates of P. aeruginosa were isolated from the patients hospitalized in the hospital from December 2017 to June 2018 in Bandar Abbas, southern Iran. MLVA of seven VNTR markers was performed for differentiating and genetic relatedness of isolates. PCR amplification was conducted for detection of blaHSV, blaVEB, int1 and int2 genes. MLVA assay with seven loci resolved 36 different genotypes. Nearly all the isolates were classified into 6 different clonal complexes and 15 singletons. High-level resistance rate was observed in ceftazidime (44; 61%). Nine (12.5%) of these isolates showed multiple drug resistance (MDR) phenotypes. Seven (9.7%), 5 (6.9%), 46 (63.8%) and 6 (8.3%) of isolates carried blaHSV, blaVEB, int1 and int2 genes, respectively. Seven VNTR markers used in this study provided a moderate discriminatory power and differentiated 36 genotypes among 72 isolates. In addition, presence of class 1 and class 2 integrons, blaHSV and blaVEB in different clo...
Source: Reviews in Medical Microbiology - Category: Microbiology Tags: BACTERIOLOGY Source Type: research