Use of plasmids for expression of proteins from the genus Leishmania in Escherichia coli: current state and perspectives.

Use of plasmids for expression of proteins from the genus Leishmania in Escherichia coli: current state and perspectives. Appl Microbiol Biotechnol. 2020 Mar 25;: Authors: Ribeiro VT, de Sá Leitão ALO, de Paiva Vasconcelos LTC, Filho MAO, Martins DRA, de Sousa Júnior FC, Dos Santos ES Abstract Leishmaniosis is caused by the protozoa of the genus Leishmania with a wide spectrum of clinical and epidemiological manifestations which are characterized into four clinical groups: cutaneous, mucocutaneous, diffuse cutaneous, and visceral. American visceral leishmaniosis (AVL) or visceral leishmaniosis (VL) has been known as the most severe form of the disease. However, despite the growing number of people exposed to the infection risk and the great effort done by the scientific community worldwide to significantly increase the knowledge about these diseases, there is no vaccine capable of preventing VL in humans. In this short review, we present some of the plasmids used for the expression of recombinant protein by Escherichia coli strains used mainly for the second generation of vaccines for leishmaniosis. It can be emphasized that currently, these vectors and hosts play an important role in developing vaccine strategies against the disease. Indeed, use of the E. coli BL21 (DE) strain is remarkable mainly due to its characteristics for being a stable protein producer as well as the use of histidine tags for antigen purification. KEY POINTS:...
Source: Applied Microbiology and Biotechnology - Category: Microbiology Authors: Tags: Appl Microbiol Biotechnol Source Type: research

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Michio Kawano1, Masaya Miyoshi1 and Tadaaki Miyazaki2* 1Milk Science Research Institute, Megmilk Snow Brand Co. Ltd., Saitama, Japan 2Department of Probiotics Immunology, Institute for Genetic Medicine, Hokkaido University, Hokkaido, Japan Lactobacillus helveticus SBT2171 (LH2171) has been reported to ameliorate the development of autoimmune diseases, such as collagen-induced arthritis and experimental autoimmune encephalitis in mice and inhibit interleukin (IL)-6 production in antigen-presenting cells in vitro. Regulation of cytokine production by antigen-presenting cells might be critical for the anti-inflamma...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
Conclusions Nucleoside hydrolases are vital enzymes for the replication of Leishmania, conserved phylogenetic marker of the genus and strong-specific immunogens. We demonstrated that NH36 is an excellent target for chemotherapy of visceral leishmaniasis. Searching for the most immunogenic fraction of promastigotes of Leishmania we described the FML antigen of L. (L.) donovani, that has as its main component, the NH36 Nucleoside hydrolase. We developed second–generation vaccines with the FML and the NH36 native antigens, and with the NH36 recombinant protein. In addition, we obtained a third generation vaccine based ...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
Marcus Vinícius Alves-Silva1,2, Dirlei Nico1, Paula Melo de Luca3 and Clarisa B. Palatnik de-Sousa1,4* 1Laboratório de Biologia e Bioquímica de Leishmania, Departamento de Microbiologia Geral, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil 2Programa de Pós-Graduação em Biotecnologia Vegetal e Bioprocessos, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil 3Laboratório de Imunoparasitologia, Instituto Oswaldo Cruz, Rio de Janeiro, Brazil 4Faculdade...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
Publication date: Available online 6 December 2018Source: PlasmidAuthor(s): Pedro J. Alcolea, Ana Alonso, Vicente LarragaAbstractDNA vaccines require a vector to replicate genes and express encoding antigens. Antibiotic resistance genes are often used as selection markers, which must not be released to the environment upon final product commercialization. For this reason, generation of antibiotic resistance-free vectors is imperative. The pPAL vector contains the cytomegalovirus enhancer and promoter for expression in mammalian cells, and the E. coli fabI chromosomal gene as a selectable marker. The fabI gene encodes the e...
Source: Plasmid - Category: Biotechnology Source Type: research
In conclusion, the results of the current study suggest that 3M2e-HSP chimer protein would be an effective universal subunit vaccine candidate against influenza infection.
Source: Virus Genes - Category: Genetics & Stem Cells Source Type: research
Publication date: September 2018Source: Meta Gene, Volume 17Author(s): Razan Abbara, Mohammad MaaroufAbstractLeishmaniasis remains one of the most neglected diseases globally and affects primarily the poor in developing countries. Due to the absence of safe and effective drug, the prevention of infection of this disease is very important as it requires an effective vaccine is not yet available. Leishmania eukaryotic initiation factor (LeIF) protein acts an inhibitor of the Leishmania major growth in murine macrophages and reduces interleukin-4 in lymph nodes. We aimed to detect its presence in Leishmania tropica genome and...
Source: Meta Gene - Category: Genetics & Stem Cells Source Type: research
Publication date: Available online 10 August 2017 Source:Journal of Bioscience and Bioengineering Author(s): Séverine Lacombe, Martine Bangratz, Jean-Paul Brizard, Elodie Petitdidier, Julie Pagniez, Drissa Sérémé, Jean-Loup Lemesre, Christophe Brugidou In recent years, plants have been shown to be an efficient alternative expression system for high-value pharmaceuticals such as vaccines. However, constitutive expression of recombinant protein remains uncertain on their level of production and biological activity. To overcome these problems, transitory expression systems have been developed. Her...
Source: Journal of Bioscience and Bioengineering - Category: Biomedical Science Source Type: research
Publication date: June 2017 Source:Microbial Pathogenesis, Volume 107 Author(s): Vinay Kumar, Shailendra Yadav, Neelagiri Soumya, Rohit Kumar, Neerupudi Kishore Babu, Sushma Singh Leishmaniasis is a group of tropical diseases caused by protozoan parasites of the genus Leishmania. Leishmania donovani is a protozoan parasite that causes visceral leishmaniasis, a fatal disease if left untreated. Chemotherapy for leishmaniasis is problematic as the available drugs are toxic, costly and shows drug resistance, hence, there is a necessity to look out for the novel drug targets, chemical entities and vaccine. Glutamine synthetase...
Source: Microbial Pathogenesis - Category: Infectious Diseases Source Type: research
CONCLUSION: Recombinant parasites were created and examined for subsequent study. With the use of molecular constructs, it was possible to remove and study gene GPI12 and to achieve a live recombinant Leishmania parasite that maintained the original form of the antigenic parasites. This achievement can be used as an experimental model for vaccine development studies. Further investigations are essential to check this model in a suitable host. PMID: 28127356 [PubMed - in process]
Source: Iranian Journal of Parasitology - Category: Parasitology Tags: Iran J Parasitol Source Type: research
da-Mansir A Abstract The demand for plasmid DNA (pDNA) has increased in response to the rapid advances in vaccines applications to prevent and treat infectious diseases caused by virus, bacteria or parasites, such as Leishmania species. The immunization protocols require large amounts of supercoiled plasmid DNA (sc-pDNA) challenging the development of efficient and profitable processes for capturing and purified pDNA molecules from large volumes of lysates. A typical bioprocess involves four steps: fermentation, primary recovery, intermediate recovery and final purification. Ion-exchange chromatography is one of t...
Source: Bioprocess and Biosystems Engineering - Category: Biomedical Engineering Authors: Tags: Bioprocess Biosyst Eng Source Type: research
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