Structural and Biomolecular Analyses of Borrelia burgdorferi BmpD Reveal a Substrate-Binding Protein of an ABC-Type Nucleoside Transporter Family Cellular Microbiology: Pathogen-Host Cell Molecular Interactions

Borrelia burgdorferi sensu lato, the causative agent of tick-borne Lyme borreliosis (LB), has a limited metabolic capacity and needs to acquire nutrients, such as amino acids, fatty acids, and nucleic acids, from the host environment. Using X-ray crystallography, liquid chromatography-mass spectrometry, microscale thermophoresis, and cellular localization studies, we show that basic membrane protein D (BmpD) is a periplasmic substrate-binding protein of an ABC transporter system binding to purine nucleosides. Nucleosides are essential for bacterial survival in the host organism, and these studies suggest a key role for BmpD in the purine salvage pathway of B. burgdorferi sensu lato. Because B. burgdorferi sensu lato lacks the enzymes required for de novo purine synthesis, BmpD may play a vital role in ensuring access to the purines needed to sustain an infection in the host. Furthermore, we show that, although human LB patients develop anti-BmpD antibodies, immunization of mice with BmpD does not confer protection against B. burgdorferi sensu lato infection.
Source: Infection and Immunity - Category: Infectious Diseases Authors: Tags: Cellular Microbiology: Pathogen-Host Cell Molecular Interactions Source Type: research

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Source: Ticks and Tick borne Diseases - Category: Zoology Source Type: research
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Source: Journal of Clinical Microbiology - Category: Microbiology Authors: Tags: Parasitology Source Type: research
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Source: The Pediatric Infectious Disease Journal - Category: Infectious Diseases Tags: Translational Medicine Reports Source Type: research
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Source: Ticks and Tick borne Diseases - Category: Zoology Source Type: research
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Source: Frontiers in cellular and infection microbiology - Category: Microbiology Source Type: research
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Source: Ticks and Tick borne Diseases - Category: Zoology Source Type: research
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Source: The Journal of Parasitology - Category: Parasitology Authors: Tags: J Parasitol Source Type: research
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