Multiplex Human Malaria Array: Quantifying Antigens for Malaria Rapid Diagnostics.

Multiplex Human Malaria Array: Quantifying Antigens for Malaria Rapid Diagnostics. Am J Trop Med Hyg. 2020 Mar 16;: Authors: Jang IK, Tyler A, Lyman C, Rek JC, Arinaitwe E, Adrama H, Murphy M, Imwong M, Proux S, Haohankhunnatham W, Barney R, Rashid A, Kalnoky M, Kahn M, Golden A, Nosten F, Greenhouse B, Gamboa D, Domingo GJ Abstract Malaria antigen detection through rapid diagnostic tests (RDTs) is widely used to diagnose malaria and estimate prevalence. To support more sensitive next-generation RDT development and screen asymptomatic malaria, we developed and evaluated the Q-Plex™ Human Malaria Array, which quantifies the antigens commonly used in RDTs-Plasmodium falciparum-specific histidine-rich protein 2 (HRP2), P. falciparum-specific lactate dehydrogenase (Pf LDH), P. vivax -specific LDH (Pv LDH), and Pan malaria lactate dehydrogenase (Pan LDH), and human C-reactive protein (CRP), a biomarker of severity in malaria. At threshold levels yielding 99.5% or more diagnostic specificity, diagnostic sensitivities against polymerase chain reaction-confirmed malaria for HRP2, Pf LDH, Pv LDH, and Pan LDH were 92.7%, 71.5%, 46.1%, and 83.8%, respectively. P. falciparum culture strains and samples from Peru indicated that HRP2 and Pf LDH combined improves detection of P. falciparum parasites with hrp2 and hrp3 deletions. This array can be used for antigen-based malaria screening and detecting hrp2/3 deletion mutants of P. falciparum. ...
Source: The American Journal of Tropical Medicine and Hygiene - Category: Tropical Medicine Authors: Tags: Am J Trop Med Hyg Source Type: research