Solubilization of Hexyl Aminolevulinate by Surfactants for Tumor Fluorescence Detection

In this study, sur factants were used to increase HAL solubility in the cell culture medium and serum, followed byin vitro fluorescence formation measurement in human pancreatic cancer cells (SW1990) andin vivo fluorescence detection in tumor ‐bearing mice. The results showed that Tween 80 (TW80) and Kolliphor® HS 15 (HS15) increased the solubility of HAL in the selected media. Although TW80 and HS15 exhibitedin vitro cytotoxicity at high concentrations (5 mg/mL), they facilitated fluorescent signal formation at the early stage of cell incubation. When surfactants were used, the FI should be determined without the routine washing process because surfactant ‐containing culture medium caused the loss of synthesized PpIX during the washing process. When HAL dissolved in TW80 solution was injected intraperitoneally into pancreatic cancer‐bearing mice at a dose of 50 mg/kg, the tumors exhibited red fluorescence, which indicated that systemic administra tion of surfactant‐solubilized HAL might be applicable for tumor fluorescence detectionin vivo.

Source: Photochemistry and Photobiology - March 4, 2020 Category: Science Authors: Lirong Ma, Xuanlin Yang, Xiuzhong Yao, Weiyu Weng Tags: RESEARCH ARTICLE Source Type: research