Monodispersed metal-based dendrimeric nanoclusters for potentiation of anti-tuberculosis action

Publication date: Available online 20 February 2020Source: Journal of Molecular LiquidsAuthor(s): Bernice Rodrigues, Pravin ShendeAbstractThe aim of the research study was to explore the applications of dendrimer complexed with copper in the form of nanoclusters with drug isoniazid by acting on M. tuberculosis H37Ra (ATCC 25177) cells. This approach resulted to minimize dosage regimen, reduce toxicity and provide site-specific action through pulmonary delivery. Copper nanoclusters were prepared using template synthesis approach that includes preparation of G 4 dendrimers using Michael addition and amidation reactions and a solvent free technique followed by addition of aqueous copper and reducing it by a reducing agent and finally loading it with isoniazid. FTIR, 1H NMR and DSC confirmed the formation of isoniazid-loaded copper nanoclusters. The entrapment of drug in the dendrimer chains showed controlled release for up to 24 h. TEM images confirmed the spherical shape and size of the clusters which is in the acceptable range for pulmonary drug delivery. A combination of isoniazid with copper nanoclusters provides a synergistic effect along with 85 μg/ml dose reduction when the activity was checked on M. tuberculosis H37Ra (ATCC 25177). Copper nanoclusters provide an alternative and an innovative therapy in the treatment of tuberculosis.Graphical abstract

Source: Journal of Molecular Liquids - February 20, 2020 Category: Molecular Biology Source Type: research