Quantitative analysis of 3’- and 6’- sialyllactose in human milk samples by HPLC-MS/MS: a validated method for the comparison of two consecutive lactation periods in the same woman

Publication date: Available online 19 February 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Orsolya Csernák, Barbara Rácz, Ágnes Alberti, Szabolcs BéniAbstractHuman milk oligosaccharides (HMOs) are complex carbohydrates that exist naturally in mother’s milk. Due to their myriad beneficial biological effects, HMOs are in the focus of current researches. Some of these complex carbohydrates are already commercially available utilizing their health-promoting benefits not just in infant formula but in dietary supplements. Among the acidic oligosaccharides 3’- and 6’-sialyllactoses (SLs) are the most abundant in human milk. Extending the health benefits of these trisaccharides, 3’-SL and 6’-SL are expected to be active ingredients in infant- and follow-on formula and novel foods, therefore reliable and easy-to-perform analytical methods are urgently needed for their characterization. Herein we report a graphitized carbon liquid chromatography-mass spectrometry-based method for the separation and quantitation of sialyllactoses in human milk. Compounds were separated as their alditol forms and were quantified using maltotriose as internal standard. The developed bioanalytical method was validated in terms of selectivity, accuracy, precision, recovery, carry-over, matrix effects and stability. Two consecutive lactation periods of the same individual were monitored for the first time and a slight decrease in SL concentrations were found during t...
Source: Journal of Pharmaceutical and Biomedical Analysis - Category: Drugs & Pharmacology Source Type: research