The effect of activated κ-opioid receptor (κ-OR) on the role of calcium sensing receptor (CaSR) in preventing hypoxic pulmonary hypertension development

Publication date: May 2020Source: Biomedicine &Pharmacotherapy, Volume 125Author(s): Juan Li, Min Jia, Manling Liu, Zhi Cao, Xinju Wang, Na Feng, Xiaoming Gu, Shumiao Zhang, Rong Fan, Haitao Guo, Yuemin Wang, Mingchao Liu, Jianming PeiAbstractκ-opioid receptor (κ-OR) plays a key role in preventing hypoxic pulmonary hypertension (HPH) development after activated by exogenous agonist U50,488H. Calcium sensing receptor (CaSR) activation induces HPH by promoting vasoconstriction and vascular remodeling. The activated κ-OR is reported to inhibit the expression of CaSR in pulmonary artery smooth muscle cells (PASMCs). Thus, in this study, we aimed to explore the effect of activated κ-OR on the role of CaSR in preventing HPH development. An HPH rat model was constructed using Sprague-Dawley rats. Changes in mean pulmonary arterial pressure (mPAP) and right ventricular pressure (RVP) mediated by κ-OR agonist U50,488H and CaSR inhibitor NPS2143 were observed. The effects of CaSR agonist spermine and inhibitor NPS2143 on pulmonary artery tension were tested. The expression and localization of κ-OR and CaSR were measured in isolated PASMCs. A cell-counting kit-8 assay was performed to evaluate the effect of spermine in PASMC proliferation. Expression of proliferating cell nuclear antigen (PCNA), Erk, and p-Erk was evaluated by western blot analysis. Results showed that κ-OR and CaSR were co-expressed and colocalized in PASMCs under normoxic ...
Source: Biomedicine and Pharmacotherapy - Category: Drugs & Pharmacology Source Type: research

Related Links:

SEPARATION from cardiopulmonary bypass (CPB) after cardiac surgery is a progressive transition from full mechanical circulatory and respiratory support to spontaneous mechanical activity of the lungs and heart. During the separation phase, measurements of cardiac performance with transesophageal echocardiography (TEE) provide the rationale behind the diagnostic and therapeutic decision-making process. In many cases, it is possible to predict a complex separation from CPB, such as when there is known preoperative left or right ventricular dysfunction, bleeding, hypovolemia, vasoplegia, pulmonary hypertension, or owing to te...
Source: Journal of Cardiothoracic and Vascular Anesthesia - Category: Anesthesiology Authors: Tags: Review Article Source Type: research
In conclusion, anti-Hp Abs are neither more frequent nor elevated in SSc compared with healthy population, the only exception being the higher frequency and levels of anti-VacA Abs in pulmonary hypertension and lung fibrosis, respectively. These results suggest that Hp is unlikely to b e involved in the development of SSc.
Source: Immunologic Research - Category: Allergy & Immunology Source Type: research
Authors: Campean IA, Lang IM Abstract Introduction: Despite an increasingly older pulmonary hypertension (PH) population, data on PH treatments in these patients are limited because there exist no clinical studies dedicated to geriatric groups. Furthermore, elderly patients with comorbidities have been systematically excluded from clinical trials, limiting the evidence base for drugs approved for pulmonary arterial hypertension (PAH).Areas covered: This review is focused on the diagnosis and treatment of pulmonary hypertension (PH) in the elderly, which is a hot topic today. Areas covered by the authors include cur...
Source: Expert Opinion on Pharmacotherapy - Category: Drugs & Pharmacology Tags: Expert Opin Pharmacother Source Type: research
SOUTH SAN FRANCISCO, CA, April 3, 2020 – Actelion Pharmaceuticals US, Inc., a Janssen Pharmaceutical Company of Johnson &Johnson, today announced that CHEST, the official publication of the American College of Chest Physicians, published results from an expert Delphi consensus survey that help provide additional insights for physicians to advance the care of patients with pulmonary arterial hypertension (PAH).The Prostacyclin International Expert Panel developed the consensus opinions based on common clinical scenarios in which they considered adding oral prostacyclin pathway agents (PPAs), including UPTRAVI®...
Source: Johnson and Johnson - Category: Pharmaceuticals Tags: Innovation Source Type: news
The recently updated European Society of Cardiology/European Respiratory Society guidelines for acute pulmonary embolism (PE) underline the importance of appropriate long-term management of PE sequelae in the era of extended anticoagulation [1]. Chronic thromboembolic disease (CTED) is one of several conditions contributing to breathlessness in this setting, i.e. persistent pulmonary vascular obstruction on imaging, with no evidence of pulmonary hypertension at rest. CTED is increasingly encountered in pulmonary vascular disease clinics following acute PE, and its diagnosis relies heavily on careful exclusion of other cond...
Source: European Respiratory Journal - Category: Respiratory Medicine Authors: Tags: Original Articles: Correspondence Source Type: research
Beyond the major gene BMPR2, several new genes predisposing to PAH have been identified during the last decade. Recently, preliminary evidence of the involvement of the KDR gene was found in a large genetic association study. We prospectively analysed the KDR gene by targeted panel sequencing in a series of 311 PAH patients referred to a clinical molecular laboratory for genetic diagnosis of PAH. Two index cases with severe PAH from two different families were found to carry a loss-of-function mutation in the KDR gene. These two index cases were clinically characterised by low diffusing capacity for carbon monoxide adjuste...
Source: European Respiratory Journal - Category: Respiratory Medicine Authors: Tags: Mechanisms of lung disease, Pulmonary vascular disease Original Articles: Pulmonary hypertension and basic science Source Type: research
The comment by C. McCabe and colleagues provides thoughtful insights into the late sequalae of pulmonary embolism. The authors' views and suggestions, based on their clinical experience and expertise, may prove helpful in the ongoing process of reliably (and meaningfully) distinguishing chronic thromboembolic pulmonary hypertension (CTEPH) from chronic thromboembolic disease (CTED) without pulmonary hypertension at rest.
Source: European Respiratory Journal - Category: Respiratory Medicine Authors: Tags: Original Articles: Correspondence Source Type: research
Authors: Sugimoto K, Yoshihisa A, Nakazato K, Yokokawa T, Misaka T, Oikawa M, Kobayashi A, Yamaki T, Kunii H, Ishida T, Takeishi Y Abstract Pulmonary hypertension (PH) caused by left-sided heart disease (LHD-PH) is classified into 2 types: isolated post-capillary PH (Ipc-PH) and combined pre- and post-capillary PH (Cpc-PH). However, the impact of pulmonary vascular resistance (PVR) or diastolic pressure gradient (DPG) on the prognosis of LHD-PH has varied among previous studies. Thus, we verified the significance of PVR or DPG on the prognosis of LHD-PH in our series.We analyzed 243 consecutive LHD-PH patients. The...
Source: International Heart Journal - Category: Cardiology Tags: Int Heart J Source Type: research
Condition:   Pulmonary Hypertension Intervention:   Radiation: Thoracic computed tomography Sponsors:   Medical University of Graz;   Ludwig Boltzmann Institute Lung Vascular Research Not yet recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
The objective of this study was to determine the prevalence of pulmonary hypertension among children with in-hospital cardiac arrest and its impact on outcomes. Design: Retrospective single-center cohort study. Setting: PICU of a quaternary care, academic children’s hospital. Patients: Children (
Source: Pediatric Critical Care Medicine - Category: Pediatrics Tags: Feature Articles Source Type: research
More News: Calcium | Drugs & Pharmacology | Hypertension | Pulmonary Hypertension | Study