Metabolite profiling of Artemisia carvifolia Buch transgenic plants and estimation of their anticancer and antidiabetic potential

Publication date: Available online 10 February 2020Source: Biocatalysis and Agricultural BiotechnologyAuthor(s): Erum Dilshad, Hammad Ismail, Mubarak Ali Khan, Rosa Maria Cusido, Bushra MirzaAbstractDiabetes and cancer are common diseases worldwide with terrific effect on human health. In the present study, a high valued medicinal plant Artemisia carvifolia Buch was subjected for evaluation of anticancer and antidiabetic potential. Previously produced rolB and rolC gene transgenic plants of A. carvifolia were also compared with the wild type plant for a differential count of cytotoxicity and antidiabetic activity. Lowest IC50 values were shown by rolB transgenic line TB4 for alpha-glucosidase (838.6 μg/ml), alpha-amylase (742.8 μg/ml) and dipeptidyl peptidase-4 (1300 μg/ml) in contrast to the wild type plant which showed IC50 values of 1608.4 μg/ml for alpha-glucosidase, 1973.8 μg/ml for alpha-amylase and 1800 μg/ml for dipeptidyl peptidase-4. Among rolC transgenic plants, TC1 showed lowest IC50 value i.e. 873 μg/ml for alpha-glucosidase, 747.7 μg/ml for alpha-amylase and 1400μ/ml for dipeptidyl peptidase-4. Viability of the cancer cells including HeLA, HePG2 and MCF7 decreased to 80% when treated with methanolic extract of the plant and it decreased to 70% when treated with n-hexane extract. Cytotoxic effect was found more enhanced when cells were treated with both the extracts combined, showing synergism between flavonoids and artemisinin. RolB tran...
Source: Biocatalysis and Agricultural Biotechnology - Category: Biotechnology Source Type: research