Differences in Biological Activities between Recombinant Human Paraoxonase 1 (rhPON1) Subtype Isozemys R/Q as Antidotes against Organophosphrus Poisoning.

In this study, we obtained two subtype isozymes of recombinant human PON1 (rhPON1), which have gene polymorphisms in 192 locis, by gene recombination. We then investigated the differences between these two recombinant isozymes (i.e. rhPON1R192 and rhPON1Q192) in their detoxification effect against organophosphorus pesticide poisoning in rats. As antidotes against chlorpyrifos poisoning, the rhPON1R192 isozyme demonstrated better effect than the rhPON1Q192 isozyme; whereas, their detoxification activities against diazinon were on the contrary. Both isozymes showed poor detoxification activity against trithion. Therefore, we concluded that if the PON1 was used as a biological scavenger against organophosphate poisoning, better detoxification effect may be achieved by selecting the PON1 subtype isozyme with higher specific hydrolytic activity. PMID: 31981688 [PubMed - as supplied by publisher]
Source: Toxicology Letters - Category: Toxicology Authors: Tags: Toxicol Lett Source Type: research