Establishment of SIAISi001-A, an induced pluripotent stem cell (iPSC) line from 66-year old Mild Cognitive Impairment (MCI) with two copies of APOE4 gene

Publication date: Available online 25 January 2020Source: Stem Cell ResearchAuthor(s): YI Yan, Yinghui Qiu, Aonan Zhao, Jian Zhao, Ying Wang, Yulei DengAbstractA 66-year old mild cognitive impairment (MCI) female patient donated her Peripheral blood mononuclear cells (PBMC). PBMC was reprogrammed using non-integrative Sendai viral vectors containing reprogramming factors OCT4, KLF4, SOX2 and C-MYC. The pluripotency of transgene-free iPSCs was confirmed by immunocytochemistry and ability of differentiation spontaneously into 3 germ layers in vitro. Moreover, the iPSC line displayed a normal karyotype. The apolipoprotein E (APOE) ε4 allele, is considered to be the strongest genetic risk factor for Sporadic Alzheimer's disease (AD). Our model might offer a good platform to study the pathological mechanisms and drug testing studies in AD and MCI.
Source: Stem Cell Research - Category: Stem Cells Source Type: research

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Source: Fight Aging! - Category: Research Authors: Tags: Newsletters Source Type: blogs
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Source: Cell and Tissue Banking - Category: Stem Cells Source Type: research
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Source: Frontiers in Pharmacology - Category: Drugs & Pharmacology Source Type: research
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Source: Fight Aging! - Category: Research Authors: Tags: Newsletters Source Type: blogs
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