Improved in vivo efficacy of clinical-grade cryopreserved human hepatocytes in mice with acute liver failure

Publication date: Available online 24 January 2020Source: CytotherapyAuthor(s): M. Teresa Donato, Miguel Bolonio, Estefanía Cabezas, María Pelechá, Eugenia Pareja, Anna Domènech, José V. Castell, M. José Gómez-lechón, Laia TolosaAbstractClinical hepatocyte transplantation short-term efficacy has been demonstrated; however, some major limitations, mainly due to the shortage of organs, the lack of quality of isolated cells and the low cell engraftment after transplantation, should be solved for increasing its efficacy in clinical applications. Cellular stress during isolation causes an unpredictable loss of attachment ability of the cells, which can be aggravated by cryopreservation and thawing. In this work, we focused on the use of a Good Manufacturing Practice (GMP) solution compared with the standard cryopreservation medium, the University of Wisconsin medium, for the purpose of improving the functional quality of cells and their ability to engraft in vivo, with the idea of establishing a biobank of cryopreserved human hepatocytes available for their clinical use. We evaluated not only cell viability but also specific hepatic function indicators of the functional performance of the cells such as attachment efficiency, ureogenic capability, phase I and II enzymes activities and the expression of specific adhesion molecules in vitro. Additionally, we also assessed and compared the in vivo efficacy of human hepatocytes cryopreserved in different media in an animal mode...
Source: Cytotherapy - Category: Cytology Source Type: research