Neuronal nicotinic acetylcholine receptor subunit and PSD-93 transcript levels decrease in male mouse MPG following cavernous nerve injury or explant culture.

Neuronal nicotinic acetylcholine receptor subunit and PSD-93 transcript levels decrease in male mouse MPG following cavernous nerve injury or explant culture. Am J Physiol Renal Physiol. 2013 Sep 18; Authors: Girard BM, Merriam LA, Tompkins JD, Vizzard MA, Parsons RL Abstract Quantitative real time-PCR was used to test whether cavernous nerve injury leads to a decrease in major pelvic ganglia (MPG) neuronal nicotinic acetylcholine receptor (nAChR) subunit and postsynaptic density-93 (PSD-93) transcript levels. Subunits α3, β4 and α7, commonly expressed in MPG, were selected for analysis. After 72 hours in explant culture, MPG transcript levels for α3, β4, α7, and PSD-93 were depressed significantly. Three days following cavernous nerve axotomy or crush in vivo, transcript levels for α3, β4 and PSD-93, but not for α7, were significantly depressed. Three days after dissecting the cavernous nerve free of underlying tissue and applying a 5 mm lateral stretch (manipulation), the transcript levels for α3 and PSD-93 also were decreased significantly. Seven days following all 3 surgical procedures, α3 transcript levels remained depressed, but PSD-93 transcript levels were still decreased only following axotomy or nerve crush. At 30 days post surgery, transcript levels for the nAChR subunits and PSD-93 had recovered. Acetylcholine-induced currents were significantly smaller in MPG neurons dissociated from 3-day explant cultured ganglia than ...
Source: Am J Physiol Renal P... - Category: Urology & Nephrology Authors: Tags: Am J Physiol Renal Physiol Source Type: research