Berberine induces dose-dependent quiescence and apoptosis in A549 cancer cells by modulating cell cyclins and inflammation independent of mTOR pathway

Publication date: Available online 22 January 2020Source: Life SciencesAuthor(s): Ravi Kumar, Mansi Awasthi, Anamika Sharma, Yogendra Padwad, Rohit SharmaAbstractAimEmerging studies have shown that application of low concentration of bioactive phytomolecules can confer anti-proliferative effects on tumour cells by inducing senescence pathways. The alkaloid berberine is recognized for its anti-cancer attributes but its potential to induce senescence in tumour cells is least understood.Materials and methodsThe present work assessed the mechanisms pertaining to dose-dependent anti-proliferative effects of berberine in the perspective of senescence and inflammation using human non-small cell lung cancer cell line (A549).Key findingsAmongst the different tested bioactive phytomolecules, berberine treatment suppressed the proliferation of A549 cells regardless of the concentration applied. Application of low doses of berberine induced a weak SA-β-gal activity and p21WAF1 expression but did not show evidence of SASP activation due to absence of NF-κB activation and expression of proinflammatory genes. However, treatment with higher dose of berberine showed no evidence of SA-β-gal activity or p21WAF1 expression, but instead induced apoptosis and suppressed the expression of cell cyclins. The proliferative capacity of berberine treated cells was at par with control cells and no SA-β-gal activity could be observed in first generation of berberine treated cells. mTO...
Source: Life Sciences - Category: Biology Source Type: research

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ConclusionsBoth pretreatment CT and CBCT radiomics features could predict disease progression and lung injury. A model with CBCT plus pretreatment CT radiomics features might improve the prediction of lung toxicity in comparison with a model with pretreatment CT features alone.Key pointsSignificant findings of the study: A model with cone ‐beam CT radiomics features plus pre‐treatment CT radiomics features might improve the prediction of lung toxicity after SBRT in stage I NSCLC patients.What this study adds: In the prediction of PFS and lung toxicity in early ‐stage NSCLC patients treated with SBRT, CBCT radiomics c...
Source: Thoracic Cancer - Category: Cancer & Oncology Authors: Tags: Original Article Source Type: research
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Source: Thoracic Cancer - Category: Cancer & Oncology Authors: Tags: ORIGINAL ARTICLE Source Type: research
BackgroundCD73 induces the dephosphorylation of adenosine monophosphate converting it to adenosine, enabling malignancies to escape from immune surveillance. Although CD73 overexpression has been reported to be a poor prognostic factor in several malignancies including non ‐small cell lung cancer (NSCLC), its predictive relevance in NSCLC patients receiving immune checkpoint inhibitors is unknown. The present research was conducted to investigate the prognostic significance of CD73 expression in NSCLC patients receiving immune checkpoint inhibitors (ICIs).MethodsWe screened 91 patients with advanced or recurrent NSCLC wh...
Source: Thoracic Cancer - Category: Cancer & Oncology Authors: Tags: ORIGINAL ARTICLE Source Type: research
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Source: Thoracic Cancer - Category: Cancer & Oncology Authors: Tags: ORIGINAL ARTICLE Source Type: research
This article reviews the efficacy of icotinib in different solid tumors with different epidermal growth factor receptor alterations.
Source: Anti-Cancer Drugs - Category: Cancer & Oncology Tags: Review Articles Source Type: research
Authors: Zhao D, Liu H, Liu H, Zhang X, Zhang M, Kolluri VK, Feng X, He Z, Wang M, Zhu T, Yan X, Zhou Y Abstract Circular RNAs (circRNAs) represent a class of endogenous non-coding RNAs. Recently, it has been reported that circRNAs might serve as novel potential biomarkers for the diagnosis of cancer, including non-small cell lung cancer (NSCLC). Mutations in the ATP binding cassette subfamily A member 3 (ABCA3) have been increasingly associated with lung disease; however, roles for circRNAs at the ABCA3 locus have not been identified. To characterize novel biomarkers in NSCLC, a bioinformatics platform was used to...
Source: American Journal of Translational Research - Category: Research Tags: Am J Transl Res Source Type: research
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Source: Archives of Medical Science - Category: General Medicine Tags: Arch Med Sci Source Type: research
Authors: Nakashima K, Saruwatari K, Sato R, Imamura K, Kajihara I, Fukusima S, Saito T, Ishizuka S, Tamanoi D, Jodai T, Hamada S, Tomita Y, Saeki S, Ichiyasu H, Oya N, Ihn H, Sakagami T Abstract Radiation recall dermatitis (RRD) is an inflammatory reaction that occurs at previously irradiated skin regions after drug administration. We herein report a patient with non-small-cell lung cancer treated previously with thoracic radiotherapy who developed severe RRD induced by atezolizumab (anti-programmed death 1 ligand 1 [PD-L1] antibody). Immunohistochemistry of the skin biopsy showed dermatitis with infiltration of CD...
Source: Internal Medicine - Category: Internal Medicine Tags: Intern Med Source Type: research
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Source: Thoracic Cancer - Category: Cancer & Oncology Authors: Tags: ORIGINAL ARTICLE Source Type: research
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Source: Cancer Medicine - Category: Cancer & Oncology Authors: Tags: ORIGINAL RESEARCH Source Type: research
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