Multilocus approach reveals discordant molecular markers and corridors for gene flow between North African populations of Fasciola hepatica

Publication date: Available online 21 January 2020Source: Veterinary ParasitologyAuthor(s): Nabil Amor, Sarra Farjallah, Paolo Merella, Abdulaziz N Alagaili, Osama B. MohammedAbstractFasciolosis is a foodborne trematodosis characterised by a worldwide distribution. Various approaches have been developed for the study of the causative agents of this parasitic infection: Fasciola hepatica, Fasciola gigantica and the aspermic intermediated forms (hybrid and introgressed). In the present study, novel and common molecular markers (pepck and pold, ITS, CO1, ND1 and CO1-trnT-rrnL) were used to characterise Fasciola flukes from the Tunisian-Algerian border, to estimate the gene flow between these populations and to evaluate the reliability of different molecular markers. All nuclear and mitochondrial markers, apart from pepck, supported the monophyly of the studied flukes identified as F. hepatica. Multiplex PCR for pepck revealed three different genotypes corresponding to F. hepatica (pepck-Fh), F. gigantica (pepck-Fg) and the aspermic Fasciola flukes (pepck-Fh/Fg). Sequence analysis of pepck revealed high polymorphism, length variation, within this intronic marker. The observed inconsistencies were due to the position of the forward primer within the intronic region. Pepck sequences showed different level of heterozygosity and homozygosity with length polymorphisms in the introns. Pepck multiplex PCR patterns could not differentiate between Fasciola species. All studies based on on...
Source: Veterinary Parasitology - Category: Veterinary Research Source Type: research

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Publication date: Available online 8 February 2020Source: Parasitology InternationalAuthor(s): Zia Ur Rehman, Osama Zahid, Imran Rashid, Qasim Ali, Muhammad Haroon Akbar, Muhammad Oneeb, Wasim Shehzad, Kamran Ashraf, Neil D. Sargison, Umer ChaudhryAbstractFasciola spp. are responsible for over 3 billion US dollars of production loss annually in livestock and cause widespread zoonotic disease. Nevertheless, understating of the emergence and spread of the trematode species is poor. The multiplicity of F. gigantica infection and its spread is potentially influenced by multiple factors, including the abundance of suitable inte...
Source: Parasitology International - Category: Parasitology Source Type: research
Fasciola hepatica, Fasciola gigantica, and Fascioloides magna are liver flukes causing disease in cattle and sheep. Damage to the liver due to F hepatica and F gigantica results in clinical disease and/or production losses. F magna seems to have little effect in cattle but causes high mortality in sheep. The fluke life cycle involves an aquatic or amphibious snail intermediate host and thus requires suitable moisture and temperature conditions. F magna requires the presence of deer. Drug treatment is the mainstay of control and needs to be applied considering the life cycle and epidemiology of the parasite.
Source: Veterinary Clinics of North America: Food Animal Practice - Category: Veterinary Research Authors: Source Type: research
eta J Abstract Fasciolosis, caused by Fasciola hepatica and Fasciola gigantica, is a globally distributed zoonotic disease of livestock. While F. hepatica and F. gigantica have temperate and tropical distributions, respectively, parasite sympatry occurs in parts of Asia and Africa. A growing protein demand has the potential to facilitate the translocation of parasites from endemic to non-endemic areas, via associated international livestock movements. Such is the case in Southeast Asia, where livestock trade from F. hepatica-endemic countries into China and Vietnam may account for detection of F. hepatica hybrid/i...
Source: International Journal for Parasitology - Category: Parasitology Authors: Tags: Int J Parasitol Source Type: research
The objective of the present work is to characterize the proteomic changes occurring in both the parasite and the host, through the development of a novel in vitro model, to shed light on the molecular pathways of communication between the newly excysted juveniles (NEJ) from F. hepatica and the host’s intestinal epithelium. For this, in vitro excystation of F. hepatica metacercariae was carried out and NEJ were obtained. Additionally, optimal conditions of growth and expansion of mouse primary small intestinal epithelial cells (MPSIEC) in culture were fine-tuned. Tegumentary and somatic parasite antigens (NEJ-Teg and...
Source: Veterinary Parasitology - Category: Veterinary Research Source Type: research
Publication date: Available online 10 January 2020Source: International Journal for Parasitology: Drugs and Drug ResistanceAuthor(s): I. Fairweather, G.P. Brennan, R.E.B. Hanna, M.W. Robinson, P.J. SkuceAbstractLiver flukes include Fasciola hepatica, Fasciola gigantica, Clonorchis sinensis, Opisthorchis spp., Fascioloides magna, Gigantocotyle explanatum and Dicrocoelium spp. The two main species, F. hepatica and F. gigantica, are major parasites of livestock and infections result in huge economic losses. As with C. sinensis, Opisthorchis spp. and Dicrocoelium spp., they affect millions of people worldwide, causing severe h...
Source: International Journal for Parasitology: Drugs and Drug Resistance - Category: Parasitology Source Type: research
This study demonstrates, for the first time, that parasitic trematode juveniles can trigger NET formation. PMID: 31917163 [PubMed - as supplied by publisher]
Source: Experimental Parasitology - Category: Parasitology Authors: Tags: Exp Parasitol Source Type: research
This study has confirmed that F. hepatica is endemic in several dairy regions in Victoria: triclabendazole resistance may be contributing to the high prevalence in some herds. From our analysis, we estimate that the state-wide economic loss associated with fasciolosis is in the order of AUD 129 million (range AUD 38-193 million) per year or about AUD 50,000 (range AUD 15,000-75,000) per herd per year.Graphical abstract
Source: Veterinary Parasitology - Category: Veterinary Research Source Type: research
Conclusion: Parasitic diseases cause important economic losses in livestock industry. In following years, it is aimed to plan prevention and control strategies for the parasites detected in this area in line with the data of this study and to share this data with the animal breeders. PMID: 31865654 [PubMed - in process]
Source: Turkish Society for Parasitology - Category: Parasitology Authors: Tags: Turkiye Parazitol Derg Source Type: research
Publication date: Available online 10 December 2019Source: Parasitology InternationalAuthor(s): L. Buffoni, M.M. Piva, P. Baska, K. Januszkiewicz, L.J. Norbury, K.C. Prior, D. Dezen, A.S. Silva, H. Wedrychowiczhz, R.E. MendesAbstractThe aim of this study was to assess the immune response and the protective efficacy elicited by the vaccination with the recombinant Fasciola hepatica myosin regulatory light chain (FhrMRLC) in Adjuplex® adjuvant against the infection with F. hepatica in rats. Four groups of 15 animals each were used for the study, one group was immunized with the recombinant F. hepatica MRLC in Adjuplex&re...
Source: Parasitology International - Category: Parasitology Source Type: research
Snails such as Galba truncatula are hosts for trematode flukes causing fascioliasis, a zoonosis that is a major public health problem. Galba truncatula has recently been shown to be a cryptic species complex. Afr...
Source: Parasites and Vectors - Category: Microbiology Authors: Tags: Research Source Type: research
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