Decellularized extracellular matrices derived from cultured cells at stepwise myogenic stages for the regulation of myotube formation

Publication date: Available online 21 January 2020Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Takashi Hoshiba, Natsumi YokoyamaAbstractThe regulation of stem cell differentiation is key for muscle tissue engineering and regenerative medicine. To this end, various substrates mimicking the native extracellular matrix (ECM) have been developed with consideration of the mechanical, topological, and biochemical properties. However, mimicking the biochemical properties of the native ECM is difficult due to its compositional complexity. To develop substrates that mimic the native ECM and its biochemical properties, decellularization is typically used. Here, substrates mimicking the native ECM at each myogenic stage are prepared as stepwise myogenesis-mimicking matrices via the in vitro myogenic culture of C2C12 myoblasts and decellularization. Cells adhered to the stepwise myogenesis-mimicking matrices at similar levels. However, the matrices derived from cells at the myogenic early stage suppressed cell growth and promoted myogenesis. This promotion of myogenesis was potentially due to the suppression of the activation of endogenous BMP signaling following the suppression of the expression of the myogenic-inhibitory factors, Id2 and Id3. Our stepwise myogenesis-mimicking matrices will be suitable ECM models for basic biological research and myogenesis of stem cells. Further, these matrices will provide insights that improve the efficacy of decell...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - Category: Molecular Biology Source Type: research

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We have identified a transcortical perivascular osteoprogenitor (transcortical perivascular cell [TPC]) located within cortical bone channels under basal conditions. When cortical bone was isolated from DMP1CreERT2/Ai9 Tomato reporter with Col2.3GFP reporter mice and transplanted into recipient mice, TPCs migrate out of the bone into the newly forming bone marrow cavity where they continue to proliferate and differentiate to form new trabecular bone. AbstractBone remodeling and regeneration are dependent on resident stem/progenitor cells with the capability to replenish mature osteoblasts and repair the skeleton. Using lin...
Source: Stem Cells - Category: Stem Cells Authors: Tags: Tissue ‐Specific Stem Cells Source Type: research
In this study, we employed a quantitative proteomics method to evaluate the impact of particle-radiation induced alterations in three major pUb-linked chains at lysine residues Lys-48 (K-48), Lys-63 (K-63) and Lys-11 (K-11), and probed for global proteomic changes in mouse and human neural cells that were irradiated with low doses of 250 MeV proton, 260 MeV/u silicon or 1 GeV/u iron ions. We found significant accumulation in K-48 linkage after 1 Gy protons and K-63 linkage after 0.5 Gy iron ions in human neural cells. Cells derived from different regions of the mouse brain (cortex, striatum and mesencephalon) showed differ...
Source: Life Sciences in Space Research - Category: Biology Source Type: research
In early research at Imperial College London, rats regrew bone mass in their broken spines. The combination of drugs boosts stem cells, which build new bone and speed up repair.
Source: the Mail online | Health - Category: Consumer Health News Source Type: news
Human-induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) have shed great light on cardiac regenerative medicine and specifically myocardial repair in heart failure patients. However, the treatment...
Source: Stem Cell Research and Therapy - Category: Stem Cells Authors: Tags: Research Source Type: research
Publication date: Available online 19 February 2020Source: Polymer Degradation and StabilityAuthor(s): Marziyeh Ranjbar Mohammadi, Saeid Kargozar, S.H. Bahrami, Shahram RabbaniAbstractIn this research, the scaffolds from gum tragacanth (GT), poly (Ɛ-caprolactone) (PCL) and poly (vinyl alcohol) (PVA), were fabricated by electrospinning method for use in the healing of diabetic ulcers. At first, three different compositions from PCL-GT-PVA with blend ratios of (2:1:0.5), (2:1:0.8) and (2:1:1.5) were fabricated and the formulation 2:1:0.8 was selected as proper one. Then, the effect of changing GT concentration on the morpho...
Source: Polymer Degradation and Stability - Category: Chemistry Source Type: research
Cancer stem cells (CSC) are a distinct subpopulation within a tumor. They are able to self-renew and differentiate and possess a high capability to repair DNA damage, exhibit low levels of reactive oxygen species (ROS), and proliferate slowly. These features render CSC resistant to various therapies, including radiation therapy (RT). Eradication of all CSC is a requirement for an effective antineoplastic treatment and is therefore of utmost importance for the patient. This makes CSC the prime targets for any therapeutic approach. Albeit clinical data is still scarce, experimental data and first clinical trials give hope th...
Source: Frontiers in Oncology - Category: Cancer & Oncology Source Type: research
Pediatric myelodysplastic syndromes (MDS) represent a spectrum of rare hematopoietic stem cell disorders characterized by cytopenias, ineffective hematopoiesis, marrow dysplasia and risk for leukemic transformation. While some cases are likely acquired due to de novo transformation, germline mutations in DNA repair pathways, ribosomal pathways, the telomere complex or in hematopoietic transcription factors (e.g., CEBPA, RUNX1, GATA2 and ETV6) are predisposition syndromes underlying many pediatric cases of MDS and acute myeloid leukemia (AML).
Source: Biology of Blood and Marrow Transplantation - Category: Hematology Authors: Source Type: research
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Source: Fight Aging! - Category: Research Authors: Tags: Medicine, Biotech, Research Source Type: blogs
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Source: Stem Cell Research - Category: Stem Cells Source Type: research
ConclusionDe-hUC-MSCs reprogramming of endogenous hSDF-1α expression may mediate the hSDF-1α/CXCR4/PI3K/Akt pathway involved in nerve repair in HIBD rats.
Source: Genes and Diseases - Category: Genetics & Stem Cells Source Type: research
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