Nepeta nuda ssp. nuda L. water extract: Inhibition of replication of some strains of Human Alpha herpes virus (genus Simplex virus) in vitro, mode of action and NMR-based metabolomics

Publication date: Available online 20 January 2020Source: Journal of Herbal MedicineAuthor(s): Anton Hinkov, Petia Angelova, Andrey Marchev, Yordan Hodzhev, Venelin Tsvetkov, Daniela Dragolova, Daniel Todorov, Kalina Shishkova, Veneta Kapchina-Toteva, Renald Blundell, Stoyan Shishkov, Milen GeorgievAbstractNepeta nuda L. has been used in traditional folk medicine for its diuretic, anti-asthmatic, antioxidant, spasmolytic, sedative and analgesic properties (attributed to the nepetalactones). In the present study, water extract from Nepeta nuda ssp. nuda L. was tested in order to study its’ effect on the replication of Human Alpha herpes virus (HHV) type 1, strain F (ACV-sensitive) and type 2, strain DD (ACV-resistant) in vitro. Toxicity was measured at 48 hours (CC50 = 7.35 mg/ml ±0.06) and 72 hours (CC50 = 4.488 mg/ml ±0.308) after infection. The extract showed potent anti-herpesvirus activity in both antiviral tests performed (MTT-based colorimetric assay and yield reduction assay). By the time the extract was added, two experimental arrangements were applied. The antiviral activity increased when water extract was added simultaneously with the inoculation of the cell monolayer (EC50 of 0.66 mg/ml ±0.04 and 0.788 mg/ml ±0.009 for the F and the DD strains, respectively, measured via colorimetric assay). The IC50 value was 0.181 mg/ml ±0.073 and 0.0888 mg/ml ±0.014 for the F...
Source: Journal of Herbal Medicine - Category: Complementary Medicine Source Type: research

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This study was carried out on 59 subjects, 16 patients with mild persistent asthma,11 patients with moderate persistent asthma, 13 patients with severe persistent asthma, and 19 age and gender matched healthy controls. The HVEM mRNA expressions of all subjects were determined by real time PCR. Correlations between HVEM mRNA expression and fractional exhaled nitric oxide (FeNO), pulmonary function test values, total blood white cell count and differential, total immunoglobulin E (IgE) level, and Asthma Control Test (ACT) score were analyzed respectively. The discrimination abilities of HVEM mRNA between different groups wer...
Source: Journal of Asthma - Category: Respiratory Medicine Tags: J Asthma Source Type: research
CONCLUSIONS: This study may provide new insights into the pathogenesis of asthma and reveal several proteins and pathways that could be exploited to develop novel treatment approaches. PMID: 32048127 [PubMed - as supplied by publisher]
Source: Biotechnology Letters - Category: Biotechnology Authors: Tags: Biotechnol Lett Source Type: research
Mepolizumab is a monoclonal antibody that inhibits IL-5 indicated as a maintenance treatment for severe eosinophilic asthma. Reactivation of the herpes zoster virus is a known adverse reaction of the drug. Here we present a patient who developed HSV transverse myelitis secondary to his mepolizumab treatment for severe asthma.
Source: Annals of Allergy, Asthma and Immunology - Category: Allergy & Immunology Authors: Source Type: research
This study examines the prevalence and patterns of influenza and herpes zoster vaccination among patients treated with mepolizumab in the real-world.
Source: Annals of Allergy, Asthma and Immunology - Category: Allergy & Immunology Authors: Source Type: research
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Source: Annals of Allergy, Asthma and Immunology - Category: Allergy & Immunology Authors: Source Type: research
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Source: Drug Safety - Category: Drugs & Pharmacology Source Type: research
You're reading 5 Surprising Things Stress Can Do to Your Body, originally posted on Pick the Brain | Motivation and Self Improvement. If you're enjoying this, please visit our site for more inspirational articles. You just got off a terrible meeting, your boss gave you an impossible deadline for the next project, your team is pushing you to make extra hours, and you still need to pick up the kids at school. Your body is in a “fight or flight response.” Your stress levels are high, you feel your breath get quicker and even feel your heart beating faster than usual. Although this is all a natural response from y...
Source: PickTheBrain | Motivation and Self Improvement - Category: Consumer Health News Authors: Tags: featured health and fitness self improvement stress Source Type: blogs
Discussion Facial nerve palsy has been known for centuries, but in 1821 unilateral facial nerve paralysis was described by Sir Charles Bell. Bell’s palsy (BP) is a unilateral, acute facial paralysis that is clinically diagnosed after other etiologies have been excluded by appropriate history, physical examination and/or laboratory testing or imaging. Symptoms include abnormal movement of facial nerve. It can be associated with changes in facial sensation, hearing, taste or excessive tearing. The right and left sides are equally affected but bilateral BP is rare (0.3%). Paralysis can be complete or incomplete at prese...
Source: PediatricEducation.org - Category: Pediatrics Authors: Tags: Uncategorized Source Type: news
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Source: Evidence-based Complementary and Alternative Medicine - Category: Complementary Medicine Tags: Evid Based Complement Alternat Med Source Type: research
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Source: American Journal of Clinical Dermatology - Category: Dermatology Source Type: research
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