Nepeta nuda ssp. nuda L. water extract: Inhibition of replication of some strains of Human Alpha herpes virus (genus Simplex virus) in vitro, mode of action and NMR-based metabolomics

Publication date: Available online 20 January 2020Source: Journal of Herbal MedicineAuthor(s): Anton Hinkov, Petia Angelova, Andrey Marchev, Yordan Hodzhev, Venelin Tsvetkov, Daniela Dragolova, Daniel Todorov, Kalina Shishkova, Veneta Kapchina-Toteva, Renald Blundell, Stoyan Shishkov, Milen GeorgievAbstractNepeta nuda L. has been used in traditional folk medicine for its diuretic, anti-asthmatic, antioxidant, spasmolytic, sedative and analgesic properties (attributed to the nepetalactones). In the present study, water extract from Nepeta nuda ssp. nuda L. was tested in order to study its’ effect on the replication of Human Alpha herpes virus (HHV) type 1, strain F (ACV-sensitive) and type 2, strain DD (ACV-resistant) in vitro. Toxicity was measured at 48 hours (CC50 = 7.35 mg/ml ±0.06) and 72 hours (CC50 = 4.488 mg/ml ±0.308) after infection. The extract showed potent anti-herpesvirus activity in both antiviral tests performed (MTT-based colorimetric assay and yield reduction assay). By the time the extract was added, two experimental arrangements were applied. The antiviral activity increased when water extract was added simultaneously with the inoculation of the cell monolayer (EC50 of 0.66 mg/ml ±0.04 and 0.788 mg/ml ±0.009 for the F and the DD strains, respectively, measured via colorimetric assay). The IC50 value was 0.181 mg/ml ±0.073 and 0.0888 mg/ml ±0.014 for the F and the DD strains, respectively, measured via yield reduction assay. Unfortuna...
Source: Journal of Herbal Medicine - Category: Complementary Medicine Source Type: research