Characterization of novel monoclonal antibodies against MERS-coronavirus spike protein.

Characterization of novel monoclonal antibodies against MERS-coronavirus spike protein. Virus Res. 2020 Jan 13;:197863 Authors: Goo J, Jeong Y, Park YS, Yang E, Jung DI, Rho S, Park U, Sung H, Park PG, Choi JA, Seo SH, Cho NH, Lee H, Lee JM, Kim JO, Song M Abstract Middle East Respiratory Syndrome coronavirus (MERS-CoV) causes severe pulmonary infection, with ∼35% mortality. Spike glycoprotein (S) of MERS-CoV is a key target for vaccines and therapeutics because S mediates viral entry and membrane-fusion to host cells. Here, four different S subunit proteins, receptor-binding domain (RBD; 358-606 aa), S1 (1-751 aa), S2 (752-1296 aa), and SΔTM (1-1296 aa), were generated using the baculoviral system and immunized in mice to develop neutralizing antibodies. We developed 77 hybridomas and selected five neutralizing mAbs by immunization with SΔTM against MERS-CoV EMC/2012 strain S-pseudotyped lentivirus. However, all five mAbs did not neutralize the pseudotyped V534A mutation. Additionally, one mAb RBD-14F8 did not show neutralizing activity against pseudoviruses with amino acid substitution of L506 F or D509 G (England1 strain, EMC/2012 L506 F, and EMC/2012 D509 G), and RBD-43E4 mAb could not neutralize the pseudotyped I529 T mutation, while three other neutralizing mAbs showed broad neutralizing activity. This implies that the mutation in residue 506-509, 529, and 534 of S is critical to generate neutraliz...
Source: Virus Research - Category: Virology Authors: Tags: Virus Res Source Type: research

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Moderna Therapeutics, a biotech company based in Cambridge, Mass., has shipped the first batches of its COVID-19 vaccine. The vaccine was created just 42 days after the genetic sequence of the COVID_19 virus, called SARS-CoV-2, was released by Chinese researchers in mid-January. The first vials were sent to the National Institute of Allergy and Infectious Diseases (NIAID), part of the National Institutes of Health (NIH) in Bethesda, MD, which will ready the vaccine for human testing as early as April. NIH scientists also began testing an antiviral drug called remdesivir that had been developed for Ebola, on a patient infec...
Source: TIME: Health - Category: Consumer Health News Authors: Tags: Uncategorized COVID-19 Source Type: news
TTP, a technology company based in Melbourn, UK, is developing a handheld PCR (polymerase chain reaction) diagnostic device that can rapidly detect influenza viruses, and one day other viruses, in samples of nasal mucus. The company claims that the s...
Source: Medgadget - Category: Medical Devices Authors: Tags: Diagnostics Exclusive Public Health Source Type: blogs
by Tridip Sardar, Indrajit Ghosh, Xavier Rod ó, Joydev Chattopadhyay Middle East Respiratory Syndrome Coronavirus (MERS-CoV) causes severe acute respiratory illness with a case fatality rate (CFR) of 35,5%. The highest number of MERS-CoV cases are from Saudi-Arabia, the major worldwide hotspot for this disease. In the absence of neither effective treatment nor a re ady-to-use vaccine and with yet an incomplete understanding of its epidemiological cycle, prevention and containment measures can be derived from mathematical models of disease epidemiology. We constructed 2-strain models to predict past outbreaks in the...
Source: PLoS Neglected Tropical Diseases - Category: Tropical Medicine Authors: Source Type: research
By Dr. Lisa Stone, Epidemiology Adviser ; Robert Salerno, Director, Global Health Security Publio Gonzalez, a biologist with the Gorgas Institute, holds a bat in Meteti, Panama, June 6, 2018, as part an Emerging Infectious Diseases Training Event (U.S. Air Force photo by Senior Airman Dustin Mullen).February 11, 2020A disease spillover event, when a virus moves from animal to human hosts, can cause significant human illness. The coronavirus (COVID-19) seems to have spilled over sometime in late 2019, at a wildlife market in Wuhan, China, leading to more than 40,000 confirmed cases and at least 910 reported deaths&nbs...
Source: IntraHealth International - Category: International Medicine & Public Health Authors: Tags: Infectious Diseases Global Health Security Source Type: news
Authors: Malik YS, Sircar S, Bhat S, Sharun K, Dhama K, Dadar M, Tiwari R, Chaicumpa W Abstract Coronaviruses are the well-known cause of severe respiratory, enteric and systemic infections in a wide range of hosts including man, mammals, fish, and avian. The scientific interest on coronaviruses increased after the emergence of Severe Acute Respiratory Syndrome coronavirus (SARS-CoV) outbreaks in 2002-2003 followed by Middle East Respiratory Syndrome CoV (MERS-CoV). This decade's first CoV, named 2019-nCoV, emerged from Wuhan, China, and declared as "Public Health Emergency of International Concern" on Ja...
Source: Veterinary Quarterly - Category: Veterinary Research Tags: Vet Q Source Type: research
aham In early December 2019 a cluster of cases of pneumonia of unknown cause was identified in Wuhan, a city of 11 million persons in the People’s Republic of China. Further investigation revealed these cases to result from infection with a newly identified coronavirus, termed the 2019-nCoV. The infection moved rapidly through China, spread to Thailand and Japan, extended into adjacent countries through infected persons travelling by air, eventually reaching multiple countries and continents. Similar to such other coronaviruses as those causing the Middle East respiratory syndrome (MERS) and severe acute resp...
Source: Viruses - Category: Virology Authors: Tags: Perspective Source Type: research
Pandemics are perversely democratic. They’re nasty, lethal and sneaky, but they don’t discriminate. No matter your age, ethnicity, religion, gender, or nation, you’re a part of the pathogenic constituency. That shared vulnerability, and the resulting human collectivism—a universal response to a universal threat—is newly and vividly evident in the face of the now-global outbreak of the novel coronavirus known as 2019-nCoV. As of writing, there have been over 30,000 diagnosed cases and over 630 related deaths. A virus that emerged in a single city, Wuhan, China—indeed, in a single crowded ...
Source: TIME: Health - Category: Consumer Health News Authors: Tags: Uncategorized 2019-nCoV Infectious Disease Source Type: news
While the threat of the new coronavirus in the United States remains limited, a network of U.S. government agencies are already furiously ramping up efforts to contain the disease, should an outbreak occur. “We are working to keep the risk low,” said Health and Human Services Secretary Alex Azar, who is leading the federal government’s response, at a press conference Friday. So far, the overwhelming number of new cases of the virus, which originated in Wuhan, China, remain in China. There are only 11 confirmed cases in the U.S. The good news, some officials and infectious disease experts tell TIME, is t...
Source: TIME: Health - Category: Consumer Health News Authors: Tags: Uncategorized Source Type: news
Once in a very rare while, a single, serious challenge consumes the attention of the entire world. We are living through one such period today as countries and communities come together to end the new coronavirus outbreak. This virus, provisionally known as the “2019-novel coronavirus,” has, as of 3 February 2020, spread to 24 countries, with over 17,000 people being infected to date, 99% of them in China. The limited numbers of cases elsewhere around the world – just 153 so far – show that efforts to contain the virus in China are curbing its global spread, alongside the detection and clinical care...
Source: TIME: Health - Category: Consumer Health News Authors: Tags: Uncategorized health ideas Source Type: news
We were surprised in 2002 when a new coronavirus called SARS emerged from southern China and spread to 17 countries, causing more than 8,000 disease cases and nearly 800 deaths. We were surprised in 2009 when a new H1N1 influenza strain emerged in Mexico and caused worldwide panic. We were surprised in 2014 when Ebola virus broke out in three West African countries, with nearly 30,000 cases and more than 11,000 deaths. And here we are now, facing the 2019-nCoV coronavirus outbreak, on the verge of becoming a worldwide pandemic, wthin China reporting over 20,000 cases and nearly 500 deaths. Three years ago in a book, Deadl...
Source: TIME: Health - Category: Consumer Health News Authors: Tags: Uncategorized 2019-nCoV health ideas Source Type: news
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