Suppressing the phenotypic virulence factors of Uropathogenic Escherichia coli using marine polysaccharide

Publication date: Available online 9 January 2020Source: Microbial PathogenesisAuthor(s): Durairajan Rubini, Prakash Vishnu Varthan, Senthilganesh Jayasankari, B. Narayanan Vedahari, Paramasivam NithyanandAbstractUropathogenic Escherichia coli (UPEC) is one of the keystone pathogen that cause 80–90% of community acquired urinary tract infections (UTIs) and Catheter associated urinary tract infections (CAUTIs). Pathogenicity and ability of UPEC to colonize the bladder majorly relies on the expression of phenotypic virulence factors like flagella, pili, curli, and non pilus adhesion. Pathogens that colonize on the indwelling medical devices are able to communicate using quorum sensing (QS) signals. QS Plays a vital role in coordinating biofilm formation which results in the bacterial cells encased inside an extracellular polymeric substance (EPS). Chitosan is a marine polysaccharide which is known for its antibacterial activity. In the present study we investigated the ability of chitosan extracted from marine biowaste to mitigate the QS mediated biofilm formation in UPEC. Extracted chitosan (EC) and Commercial chitosan (CC) showed percentage inhibition of 80–85% and 60–75% respectively on young biofilm inhibition and preformed biofilm disruption. EC and CC were assessed for its ability to suppress QS mediated virulence in UPEC. Hemolysis assay showed a percentage inhibition of 79% against EC. Both chitosan showed profound activity to suppress the phenotypic virulence fac...
Source: Microbial Pathogenesis - Category: Infectious Diseases Source Type: research