The immobilization of fibronectin- and fibroblast growth factor 2-derived peptides on a culture plate supports the attachment and proliferation of human pluripotent stem cells

Publication date: Available online 7 January 2020Source: Stem Cell ResearchAuthor(s): Ahmed Abdal Dayem, Jihye Won, Hui-Gwan Goo, Gwang-Mo Yang, Dong Sik Seo, Byeong-Min Jeon, Hye Yeon Choi, Sang Eun Park, Kyung Min Lim, Seon-Ho Jang, Soo Bin Lee, Sang Baek Choi, Kyeongseok Kim, Geun-Ho Kang, Gyu-Bum Yeon, Dae-Sung Kim, Ssang-Goo ChoAbstractPluripotent stem cells (PSCs) offer a promising tool for regenerative medicine. The clinical application of PSCs inevitably requires a large-scale culture in a highly defined environment. The present study aimed to devise defined coating materials for the efficient adhesion and proliferation of human PSCs (hPSCs). We tested the activity of seven fibronectin-derived peptides and three laminin-derived peptides for the attachment and proliferation of hPSCs through their immobilization on the bottom of culture dishes by creating a fusion protein with the mussel adhesion protein. Among the extracellular matrix (ECM) mimetics tested, one fibronectin-derived peptide, PHSRN-GRGDSP, significantly promoted adhesion, enhanced alkaline phosphatase activity, and increased pluripotency-related gene expression in hPSCs compared to Matrigel. Furthermore, co-immobilization of a particular canofin peptide derived from fibroblast growth factor 2 increased pluripotency marker expression, which may offer the possibility of culture without growth factor supplementation. Our findings afford a novel defined condition for the efficient culture of hPSCs and may be ...
Source: Stem Cell Research - Category: Stem Cells Source Type: research