Ultrastructural Characteristics of DHA-Induced Pyroptosis

This study used scanning electron microscopy (SEM) and transmission electron microscopy (TEM) to investigate the morphological characteristics of pyroptosis in BV-2 microglial cells following exposure to 200  µM DHA. Vehicle-treated cells are characterized by extended processes, spine-like projections or 0.4 to 5.2 µm in length, and numerous extracellular vesicles (EVs) tethered to the surface of the plasma membrane. In contrast to vehicle-treated cells, gross abnormalities are observed after treati ng cells with 200 µM DHA for 4 h. These include the appearance of numerous pits or pores of varying sizes across the cell surface, structural collapse and flattening of the cell shape. Moreover, EVs and spines were lost following DHA treatment, possibly due to release from the cell surface. The m embrane pores appear after DHA treatment initially measured ~ 30 nm, consistent with the previously reported gasdermin D (GSDMD) pore complexes. Complete collapse of cytoplasmic organization and loss of nuclear envelope integrity were also observed in DHA-treated cells. These processes are morp hologically distinct from the changes that occur during cisplatin-induced apoptosis, such as the appearance of apoptotic bodies and tightly packed organelles, and the maintenance of EVs and nuclear envelope integrity. Cumulatively, this study provides a systematic description of the ultrastructural characteristics of DHA-induced pyroptosis, i...
Source: NeuroMolecular Medicine - Category: Neurology Source Type: research

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Authors: Akhter MH, Kumar S, Nomani S Abstract Objective: In vitro, optimization, characterization and cytotoxic studies of NAR NPs to against pancreatic cancer.Method: The sonication tailored NARG loaded poly (lactide-co-glycolic acid) (PLGA) nanoparticle (NPs) was fabricated for potential cytotoxic effect against pancreatic cancer. NARG NPs were prepared by emulsion-diffusion evaporation technique applying Box Behnken experimental design based on 3-level and 3-factors. The effect of independent variables surfactant concentration (X1), polymer concentration (X2) and sonication time (X3) were studied on responses p...
Source: Drug Development and Industrial Pharmacy - Category: Drugs & Pharmacology Tags: Drug Dev Ind Pharm Source Type: research
Conclusions: Although 3D printed PSTs help to avoid the misplacement of PAs in revision deformity correction surgeries with sublaminar wires in situ, the mean number of misplaced screws per patient using this technique was found to be statistically insignificant when compared with the freehand technique in this study. PMID: 32213799 [PubMed - as supplied by publisher]
Source: Asian Spine Journal - Category: Orthopaedics Tags: Asian Spine J Source Type: research
Conclusions: In this study, the SUV of 99mTc-MDP was successfully determined using SPECT/CT. This research provides an approach that could potentially aid in the clinical quantification of radionuclide uptake in normal vertebrae for the management of breast cancer patients. PMID: 32213791 [PubMed - as supplied by publisher]
Source: Asian Spine Journal - Category: Orthopaedics Tags: Asian Spine J Source Type: research
Peripheral blood smear for patients with CLL and AIHA usually shows lymphoid cells with scant cytoplasm and small round nuclei with condensed chromatin, smudge cells and spherocytes. AbstractPeripheral blood smear for patients with CLL and AIHA usually shows lymphoid cells with scant cytoplasm and small round nuclei with condensed chromatin, smudge cells and spherocytes.
Source: Clinical Case Reports - Category: General Medicine Authors: Tags: CLINICAL IMAGE Source Type: research
The country has drawn global attention with an unorthodox approach while its neighbors have imposed extensive restrictions.
Source: NYT Health - Category: Consumer Health News Authors: Tags: Sweden Denmark Norway Coronavirus (2019-nCoV) Politics and Government Shutdowns (Institutional) Epidemics Source Type: news
Now, more than ever, people need tools to handle their emotions. Specifically, we need tools for calming our nervous system down and making sure we don’t take stress out on our own loved ones. Families are going to fight more when they’re cooped up. The best thing we can do for our relationships is become aware of our emotions and learn skills to calm them so we don’t behave too badly. “Every couple I know said they fought after going to the grocery store last week. Even one that went separately. That’s when I picked a fight with my boyfriend. And it makes sense because the grocery store scene...
Source: World of Psychology - Category: Psychiatry & Psychology Authors: Tags: Anger Family Self-Help coronavirus COVID-19 pandemic quarantine Source Type: blogs
CONCLUSION: PET/CT is a useful tool in the evaluation of colorectal cancer, and it allows to metabolically characterize undetermined lesions suspected for recurrence of disease, to perform a complete pre-surgical staging and to identify occult metastatic disease. PET/CT should be considered an essential diagnostic tool in the management of patients with colorectal cancer, especially in the preoperative staging. KEY WORDS: Colorectal Cancer, Computed tomography, Magnetic resonance imaging, Positron emission tomography. PMID: 32213686 [PubMed - as supplied by publisher]
Source: Annali Italiani di Chirurgia - Category: Surgery Tags: Ann Ital Chir Source Type: research
CONCLUSIONS: There is no single absolute modality for de tecting ALNMs in breast cancers to replace sentinel lymph node biopsy or axillary lymph node dissection. If ALNM is suspected based on PET/CT, axillary lymph node dissection without sentinel lymph node biopsy might be a better option because it is related to high possibilities of ALNM. KEY WORDS: Axillary lymph node metastasis, Magnetic resonance imaging, Ultrasonography, 18F-FDG PET/CT. PMID: 32213685 [PubMed - as supplied by publisher]
Source: Annali Italiani di Chirurgia - Category: Surgery Tags: Ann Ital Chir Source Type: research
L ‐2‐[18F]fluoro ‐alpha‐methylphenylalanine (2‐[18F]FAMP) is a promising amino acid tracer for positron emission tomography (PET) imaging, yet the low production yield of direct electrophilic radiofluorination with [18F]F2 necessitates further optimization of the radiolabeling process. This paper describes a 2 ‐step preparation method for L‐2‐[18F]fluoro ‐alpha‐methylphenylalanine (2‐[18F]FAMP) starting from [18F]fluoride.The (Mesityl)(L ‐alpha‐methylphenylalanine)‐2‐iodonium tetrafluoroborate precursors with various protecting groups were prepared. The copper‐mediated18F ‐fluorination of ...
Source: Journal of Labelled Compounds and Radiopharmaceuticals - Category: Biochemistry Authors: Tags: RESEARCH ARTICLE Source Type: research
Authors: Nishibe T, Nishibe M, Akiyama S, Nukaga S, Ogino H, Koizumi J, Dardik A Abstract BACKGROUND: To quantitatively evaluate edema of the lower legs in patients with saphenous varicose veins, and to determine the association between leg edema and venous hemodynamics of the lower legs. METHODS: The data of 140 patients with saphenous varicose veins visiting Eniwa Midorino Clinic from April 2018 to November 2019 were retrospectively analyzed. Body composition values, including mass index (BMI), extracellular water / total body water (ECW/TBW) of each leg, and skeletal muscle mass of each leg, were measured by...
Source: International Angiology - Category: Cardiology Tags: Int Angiol Source Type: research
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