Promotion on NLRC5 upregulating MHC-I expression by IFN- γ in MHC-I–deficient breast cancer cells

In this study, qRT-PCR, western blot, and flow cytometry were used to detect the mRNAs and proteins of NLRC5, β2m, and HLA-ABC in MHC class I–deficient human SKBR3 breast cancer cells after I FN-γ treatment. It was shown that the relative levels of NLRC5 mRNA, β2m mRNA, and HLA-ABC α heavy chain mRNA, in concentrations of 50 U/ml and 100 U/ml IFN-γ groups, were statistically increased (p <  0.05) with dose dependent tendency compared with the control group. The protein levels of NLRC5 and β2m in concentrations of 50 U/ml and 100 U/ml IFN-γ groups, HLA-ABC (positive rates) in different concentrations of IFN-γ groups, were statistically increased (p <  0.05), with dose dependent tendency for NLRC5 and HLA-ABC, compared with the control group. Promotion of NLRC5 by IFN-γ with upregulation of MHC class I (HLA-ABC) in SKBR3 breast cancer cells, suggesting the contribution to counteracting cancer evasion from immunosurveillance and benefiting canc er immunotherapy.
Source: Immunologic Research - Category: Allergy & Immunology Source Type: research