Blistering1 modulates Penicillium expansum virulence via vesicle-mediated protein secretion.

Blistering1 modulates Penicillium expansum virulence via vesicle-mediated protein secretion. Mol Cell Proteomics. 2019 Dec 23;: Authors: Jurick WM, Peng H, Beard HS, Garrett WM, Lichtner FJ, Luciano-Rosario D, Macarisin O, Liu Y, Peter KA, Gaskins VL, Yang T, Mowery J, Bauchan G, Keller N, Cooper B Abstract The blue mold fungus, Penicillium expansum, is a postharvest apple pathogen that contributes to food waste by rotting fruit and by producing harmful mycotoxins (e.g. patulin). To identify genes controlling pathogen virulence, a random T-DNA insertional library was created from wild-type P. expansum strain R19. One transformant, T625, had reduced virulence in apples, blistered mycelial hyphae, and a T-DNA insertion that abolished transcription of the single copy locus in which it was inserted.  The gene, Blistering1, encodes a protein with a DnaJ domain, but otherwise has little homology outside the Aspergillaceae, a family of fungi known for producing antibiotics, mycotoxins, and cheese. Because protein secretion is critical for these processes and for host infection, mass spectrometry was used to monitor proteins secreted into liquid media during fungal growth. T625 failed to secrete a set of enzymes that degrade plant cell walls, along with ones that synthesize the three final biosynthetic steps of patulin.  Consequently, the culture broth of T625 had significantly reduced capacity to degrade apple tissue and contained 30 time...
Source: Molecular and Cellular Proteomics : MCP - Category: Molecular Biology Authors: Tags: Mol Cell Proteomics Source Type: research