Application of an optically induced dielectrophoresis (ODEP)-based microfluidic system for the detection and isolation of bacteria with heterogeneity of antibiotic susceptibility

Publication date: Available online 5 December 2019Source: Sensors and Actuators B: ChemicalAuthor(s): Hsin-Yao Wang, Chih-Yu Chen, Po-Yu Chu, Yu-Xian Zhu, Chia-Hsun Hsieh, Jang-Jih Lu, Min-Hsien WuAbstractDifferent levels of antibiotic resistance reside in a bacterial clone (heteroresistance) is regarded as an important cause leading to development of superbugs and treatment failure of infectious diseases. However, phenotypical detection of bacterial heteroresistance is not available in current clinical practice or by present analytical tools. To address this issue, we developed an optically induced dielectrophoresis (ODEP)-based microfluidic system capable of detecting bacterial heteroresistance and isolating minor resistant strain within a bacterial clone. The working principle is based on the different levels of ODEP force exerted on the bacteria with different degrees of viability, which was resulted from different degrees of antibiotic resistance. We designed and fabricated an ODEP-based microfluidic system, and used standard bacterial strains, namely E. coli ATCC 35218 (antibiotic resistant strain) and E. coli ATCC 25922 (antibiotic susceptible strain) to prove the proposed concept. After treated with ampicillin for 1 hour, we demonstrated that both bacterial strains could be isolated with high recovery rate up to 98.07%. Moreover, we mixed E. coli ATCC 35218 and E. coli ATCC 25922 with a variety of heterogeneous ratios to simulate heteroresistance. The ratio of the r...
Source: Sensors and Actuators B: Chemical - Category: Chemistry Source Type: research