Application of an optically induced dielectrophoresis (ODEP)-based microfluidic system for the detection and isolation of bacteria with heterogeneity of antibiotic susceptibility

Publication date: Available online 5 December 2019Source: Sensors and Actuators B: ChemicalAuthor(s): Hsin-Yao Wang, Chih-Yu Chen, Po-Yu Chu, Yu-Xian Zhu, Chia-Hsun Hsieh, Jang-Jih Lu, Min-Hsien WuAbstractDifferent levels of antibiotic resistance reside in a bacterial clone (heteroresistance) is regarded as an important cause leading to development of superbugs and treatment failure of infectious diseases. However, phenotypical detection of bacterial heteroresistance is not available in current clinical practice or by present analytical tools. To address this issue, we developed an optically induced dielectrophoresis (ODEP)-based microfluidic system capable of detecting bacterial heteroresistance and isolating minor resistant strain within a bacterial clone. The working principle is based on the different levels of ODEP force exerted on the bacteria with different degrees of viability, which was resulted from different degrees of antibiotic resistance. We designed and fabricated an ODEP-based microfluidic system, and used standard bacterial strains, namely E. coli ATCC 35218 (antibiotic resistant strain) and E. coli ATCC 25922 (antibiotic susceptible strain) to prove the proposed concept. After treated with ampicillin for 1 hour, we demonstrated that both bacterial strains could be isolated with high recovery rate up to 98.07%. Moreover, we mixed E. coli ATCC 35218 and E. coli ATCC 25922 with a variety of heterogeneous ratios to simulate heteroresistance. The ratio of ...
Source: Sensors and Actuators B: Chemical - Category: Chemistry Source Type: research

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Publication date: Available online 23 January 2020Source: Process BiochemistryAuthor(s): Prakashkumar Nallasamy, Thenmozhi Ramalingam, Nooruddin Thajuddin, Rajasree Shanmuganathan, Arivalagan Pugazhendhi, Suganthy NatarajanAbstractStarch is a natural, renewable biopolymer widely used in pharmaceutical industry for the controlled release of drugs and hormones. Triphala Churna (TC) a tridoshic rasayana, rich in polyphenols and vitamin C possess balancing and rejuvenating effect on three constitutional elements that govern the human life. To enhance the solubility and efficiency of the ayurvedic drug TC, attempts were made to...
Source: Process Biochemistry - Category: Biochemistry Source Type: research
(University of Gothenburg) A hitherto unknown antibiotic-resistant bacteria species, in the same family as E. coli and Salmonella spp., has been found and classified in Sweden. The proposed taxonomic name of the species -- the first of the new genus -- is Scandinavium goeteborgense, after the city of Gothenburg, Sweden, where the bacterium was isolated and the research was done.
Source: EurekAlert! - Infectious and Emerging Diseases - Category: Infectious Diseases Source Type: news
Poultry husbandry is important for the economic health of Pakistan, but the Pakistani poultry industry is negatively impacted by infections from Escherichia coli. We performed Illumina whole genome sequencing on 92 E. coli isolates obtained from the livers of deceased chickens originating in five Pakistani geographical regions. Our analysis indicates that the isolates are predominantly from the B1 and A clade and harbor a diverse number of antibiotic resistance and virulence genes, with no linkage between phylogeny and antibiotic resistance gene presence but some association between phylogeny and virulence gene and SNP pre...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Publication date: 1 March 2020Source: Materials Chemistry and Physics, Volume 243Author(s): Samir Bouchareb, Rachida Doufnoune, Farid Riahi, Hafsa Cherif-Silini, Lassaad BelbahriAbstractGO-Ag nanohybrids have attracted tremendous attention thanks to their several potential properties. In this research work, a new approach was adopted, where Triphenylphosphine (PPh3) was used as a linkage to decorate the surface of Graphene oxide (GO) nanosheets by Silver nanoparticles (AgNPs) via a simple method. The Fourier transform infrared spectroscopy (FTIR), Ultraviolet–visible spectroscopy (UV–Vis), X-ray diffraction ana...
Source: Materials Chemistry and Physics - Category: Materials Science Source Type: research
This study characterized presumptive E. coli isolated from Obushera, a traditional fermented cereal beverage from Uganda. Thereafter, the antimicrobial effect of lactic acid bacteria (LAB) previously isolated from Obushera, against the E. coli, was examined. The presumptive E. coli was incubated in brain heart infusion broth (pH = 3.6) at 25°C for 48 h. The most acid-stable strains were clustered using (GTG)5 rep-PCR fingerprinting and identified using 16S rRNA sequencing. E. coli was screened for Shiga toxins (Stx 1 and Stx 2) and Intimin (eae) virulence genes as well as antibiotic resistance. The...
Source: International Journal of Microbiology - Category: Microbiology Tags: Int J Microbiol Source Type: research
In this study, we presented two different expression systems for the production of PR-39 in E. coli; one in fusion with Intein-Chitin binding domain (CBD) and another in fusion with SUMO accompanied by polyhistidine affinity tag. Both were cloned in the NdeI-XhoI sites of pET-17b and transformed to E. coli BL21 (DE3) pLysS. Recombinant bacteria were cultured and induced with 0.4 mM IPTG at 30 °C. Expression and purification of target proteins were confirmed by Tricine- SDS-PAGE and dot blot analysis. Recovery of 250 μg PR-39/L from SUMO fusion system and 280 μg PR-39/L from the intein fusion system was achieved.
Source: Protein Expression and Purification - Category: Biochemistry Source Type: research
Publication date: Available online 11 January 2020Source: Materials Science and Engineering: CAuthor(s): Xiumei Zhen, Lalit Chudal, Nil Kanatha Pandey, Jonathan Phan, Xin Ran, Eric Amandor, Xuejing Huang, Omar Johnson, Yuping Ran, Wei Chen, Michael R. Hamblin, Liyi HuangAbstractHerein, for the first time, we demonstrate that the combination of copper-cysteamine (Cu-Cy) nanoparticles (NPs) and potassium iodide (KI) can significantly inactivate both Gram-positive MRSA and Gram-negative E. coli. To uncover the mystery of the killing, the interaction of KI with Cu-Cy NPs was investigated systematically and the products from th...
Source: Materials Science and Engineering: C - Category: Materials Science Source Type: research
Cecilia Ambrosi Urinary tract infections (UTIs) are mainly caused by uropathogenic Escherichia coli (UPEC). Acute and recurrent UTIs are commonly treated with antibiotics, the efficacy of which is limited by the emergence of antibiotic resistant strains. The natural sugar d-mannose is considered as an alternative to antibiotics due to its ability to mask the bacterial adhesin FimH, thereby preventing its binding to urothelial cells. Despite its extensive use, the possibility that d-mannose exerts “antibiotic-like” activity by altering bacterial growth and metabolism or selecting FimH variants has no...
Source: Molecules - Category: Chemistry Authors: Tags: Article Source Type: research
Trusts and clinical commissioning groups face new annual targets designed to combat rising levels of four bloodstream infections, but sanctions relating to MRSA and C difficile could be scrapped.
Source: HSJ - Category: UK Health Source Type: news
Abstract The search for new, powerful antimicrobials is essential to respond to the current worldwide spread of antibiotic-resistant pathogens. Sponge-associated bacteria have great potential for production of antimicrobials against resistant and multidrug resistant (MDR) pathogenic bacteria, but only few species of the Class Homoscleromorpha have been screened for these activities so far. The aim of this study was to isolate and identify sponge-associated bacteria active against antibiotic-resistant pathogens from sponges of classes Homoscleromorpha and Demospongiae. By employin...
Source: Current Microbiology - Category: Microbiology Authors: Tags: Curr Microbiol Source Type: research
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