Isolation of bacteria at different points of Pleurotus ostreatus cultivation and their influence in mycelial growth

Publication date: Available online 13 December 2019Source: Microbiological ResearchAuthor(s): Christian Suarez, Stefan Ratering, Victoria Weigel, Julia Sacharow, Jackeline Bienhaus, Janine Ebert, Anika Hirz, Martin Rühl, Sylvia SchnellABSTRACTPleurotus ostreatus is one of the most cultivated edible mushrooms worldwide and few approaches have been done to analyze bacterial influence during its cultivation. Therefore, bacteria from commercial spawn, mycelial-colonized straw and fruiting bodies from healthy productive samples were counted, isolated and tested for their mycelial growth promoting ability. Bacterial cell numbers at different steps of the process showed low bacterial cell numbers in spawn and in fruiting bodies inner tissue compared to the high concentration in mycelial-colonized straw. The majority of the 38 isolates belonged to phyla Firmicutes and Actinobacteria were identified as Bacillus, Paenibacillus and Micromonospora species. Similarly, 16S rRNA gene bacterial clones obtained from mycelial biomass DNA samples showed bacterial presence of various genera including Bacillus and Paenibacillus.In the mycelial growth promoting ability tests, 30 isolates negatively affected mycelial growth, two isolates showed no effect on mycelial growth, and six isolates promoted mycelial growth. Moreover, mycelial thickness was influenced in different ways by the bacterial growth. In general, nearly all isolates growth-preventing were isolated from healthy spawn and mycelial-c...
Source: Microbiological Research - Category: Infectious Diseases Source Type: research