Level of seven neuroblastoma-associated mRNAs detected by droplet digital PCR is associated with tumor relapse/regrowth of high-risk neuroblastoma patients

Monitoring of several sets of neuroblastoma-associated mRNAs (NB-mRNAs) by quantitative PCR (qPCR) can be used for evaluating minimal residual disease (MRD) in neuroblastoma (NB) patients. Droplet digital PCR (ddPCR) is an adaption of qPCR that potentially provides more simple and reproducible detection of low-level of mRNAs. However, it remains tested whether MRD in NB patients can be monitored by ddPCR using a set of NB-mRNAs. In the present study, 208 bone marrow (BM) and 67 peripheral blood (PB) samples were retrospectively collected from 20 high-risk NB patients with clinical disease evaluation at two Japanese centers between 2011 and 2018, and level of each NB-mRNA (CRMP1, DBH, DDC, GAP43, ISL1, PHOX2B, and TH mRNA) was determined by ddPCR.

Source: Journal of Molecular Diagnostics - December 10, 2019 Category: Pathology Authors: Khin Kyae Mon Thwin, Toshiaki Ishida, Suguru Uemura, Nobuyuki Yamamoto, Kyaw San Lin, Akihiro Tamura, Aiko Kozaki, Atsuro Saito, Kenji Kishimoto, Takeshi Mori, Daiichiro Hasegawa, Yoshiyuki Kosaka, Nanako Nino, Satoru Takafuji, Kazumoto Iijima, Noriyuki N Tags: Regular Article Source Type: research