Identification and innate immunity mechanism of protective immunogens from extracellular proteins of Edwardsiella tarda

Publication date: Available online 10 December 2019Source: Fish &Shellfish ImmunologyAuthor(s): Lu Li, Ming Song, Bo Peng, Xuan-xian Peng, Hui LiAbstractOne of the most important emerging pathogens in the aquaculture industry is Edwardsiella tarda, and it causes extensive losses in farmed fish globally. The identification of protective immunogens against E. tarda is increasingly valued. We previously investigated 20 recombinant proteins of 38 E. tarda extracellular secretory proteins and identified 10 as protective immunogens in a zebrafish model. Here, we clone 10 of the remaining 18 genes, and the resulting recombinant proteins are used for evaluation of immune protection. ETAE_2147 (FliK), ETAE_0654 (PpdD), and ETAE_3259 (DamX) are identified as protective immunogens. Furthermore, their protection mechanism is explored by the detection of innate immunity genes encoding IL-1b, IL-6, IL-8, C3b, and NF-κB. The three protective immunogens stimulate zebrafish to produce higher and more lasting expression of the five immunity genes than non-protective immunogens during the first 48 h of infection. In addition, these protective immunogens are prone to be regulated by host products, which is helpful for cross-talk between host and pathogen, and thus they become vaccine candidates. These results highlight the way to understand the working mechanisms of protective immunogens.
Source: Fish and Shellfish Immunology - Category: Biology Source Type: research

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Source: Ticks and Tick borne Diseases - Category: Zoology Source Type: research
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Source: Veterinary Parasitology - Category: Veterinary Research Source Type: research
Publication date: Available online 21 January 2020Source: American Journal of Kidney DiseasesAuthor(s): Dana Miskulin, Daniel E. Weiner, Harold J. Manley
Source: American Journal of Kidney Diseases - Category: Urology & Nephrology Source Type: research
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Source: American Journal of Kidney Diseases - Category: Urology & Nephrology Source Type: research
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Source: Psychology, Health and Medicine - Category: International Medicine & Public Health Authors: Source Type: research
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Source: American Journal of Preventive Medicine - Category: International Medicine & Public Health Source Type: research
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Source: CNN.com - Health - Category: Consumer Health News Source Type: news
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Source: Multiple Sclerosis and Related Disorders - Category: Neurology Source Type: research
An Ohio doctor who received a flood of threats over a pro-vaccine social media post is standing by her message. In a playful Tik Tok video, Dr. Nicole Baldwin showed the benefits of vaccines, and said they do not cause autism. But that prompted a barrage of hateful responses. Despite scientific evidence that there ’s no link between vaccines and autism, a recent poll found 46% of parents are still unsure. Dr. Jon LaPook joins “CBS This Morning” to discuss how doctors can counter misinformation about vaccines.
Source: Health News: CBSNews.com - Category: Consumer Health News Source Type: news
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Source: AllAfrica News: Health and Medicine - Category: African Health Source Type: news
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