Histone acetylation together with DNA demethylation empowers higher plasticity in adipocytes to differentiate into osteoblasts.

Histone acetylation together with DNA demethylation empowers higher plasticity in adipocytes to differentiate into osteoblasts. Gene. 2019 Dec 03;:144274 Authors: Cho YD, Kim BS, Kim WJ, Kim HJ, Baek JH, Woo KM, Seol YJ, Ku Y, Ryoo HM Abstract Bone regeneration has been a challenge for both researchers and clinicians. In the field of tissue engineering, much effort has been made to identify cell sources including stem cells. The present study aimed to induce trans-differentiation from adipocytes to osteoblasts using epigenetic modifiers; 5-aza-dC and/or trichostatin-A (TSA). 3T3-L1 preadipocytes were treated with TSA (100 nM) and then with Wnt3a (50 ng/ml). Microscopic observation showed trans-differentiated cell morphology. Methylation-specific PCR and immunoblotting were performed to analyze the DNA methylation and histone acetylation patterns. The gene expression was determined by real-time PCR. Based on these in vitro experiments, in vivo mouse experiments supplemented the possibility of trans-differentiation by epigenetic modification. TSA induced the acetylation of lysine9 on histone H3, and a sequential Wnt3a treatment stimulated the expression of bone marker genes in adipocytes, suppressing adipogenesis and stimulating osteogenesis. Furthermore, TSA induced DNA hypomethylation, and a combined treatment with TSA and 5-aza-dC showed a synergistic effect in epigenetic modifications. The number of adipocytes and DNA methylation p...
Source: Gene - Category: Genetics & Stem Cells Authors: Tags: Gene Source Type: research