Characterization and discrimination of microbial community and co ‐occurrence patterns in fresh and strong flavor style flue‐cured tobacco leaves

In this study, we use 16S and 18S rRNA Illumina MiSeq sequencing data to characterize and compare the microbiota associated with fresh and strong flavor style flue ‐cured tobacco leaves and reveal correlations between the microbial taxa within them. Flue‐cured tobacco leaf microbiota are correlated with flavor style, and species present at low numbers within the microbial communities can be used as microbial markers to discriminate between samples of diffe rent flavor styles and to stabilize flue‐cured tobacco leaf microbial communities. AbstractFermentation, also known as aging, is vital for enhancing the quality of flue ‐cured tobacco leaves (FTLs). Aged FTLs demonstrate high‐quality sensory characteristics, while unaged FTLs do not. Microbes play important roles in the FTL fermentation process. However, the eukaryotic microbial community diversity is poorly understood, as are microbial associations within FTLs . We aimed to characterize and compare the microbiota associated with two important categories, fresh and strong flavor style FTLs, and to reveal correlations between the microbial taxa within them. Based on 16S and 18S rRNA Illumina MiSeq sequencing, the community richness and diversity of prokaryo tes were almost as high as that of eukaryotes. The dominant microbes of FTLs belonged to seven genera, includingPseudomonas, Bacillus,Methylobacterium,Acinetobacter,Sphingomonas,Neophaeosphaeria, andCladosporium, of theProteobacteria,Firmicutes, andAscomycota ph...
Source: MicrobiologyOpen - Category: Microbiology Authors: Tags: ORIGINAL ARTICLE Source Type: research

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In its native environment of rotting vegetation, the soil nematode Caenorhabditis elegans encounters a range of bacteria. This includes species from the ESKAPE group of pathogens that pose a clinical problem in acquired hospital infections. Here, we investigated three Gram-negative members of the ESKAPE group, Pseudomonas aeruginosa, Klebsiella pneumoniae, and Acinetobacter baumannii. Pathogenicity profiles as measured by time to kill adult C. elegans showed that P. aeruginosa was the most pathogenic, followed by K. pneumoniae, while C. elegans cultured on A. baumannii exhibited the same survival as those on the standard l...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
AbstractAcinetobacter baumannii is well known for causing hospital ‐associated infections due in part to its intrinsic antibiotic resistance as well as its ability to remain viable on surfaces and resist cleaning agents. In a previous publication,A. baumannii strain AB5075 was studied by transposon mutagenesis and 438 essential gene candidates for growth on rich ‐medium were identified. The Seattle Structural Genomics Center for Infectious Disease entered 342 of these candidate essential genes into our pipeline for structure determination, in which 306 were successfully cloned into expression vectors, 192 were detectab...
Source: Protein Science - Category: Biochemistry Authors: Tags: Article Source Type: research
jamin Parkinson We aimed to describe the epidemiology, multi-drug resistance and seasonal distribution of bacteria cultured within 12 months following lower limb orthopaedic surgery in tropical and subtropical Australian hospitals between 2010 and 2017. We collected data from four tropical and two subtropical hospitals. Categorical variables were examined using the Pearson Chi-squared test or Fisher’s Exact test, and continuous variables with the Student t-test or Mann–Whitney U test. A Poisson regression model was used to examine the relationship between season, weather and the incidence of Staphyl...
Source: International Journal of Environmental Research and Public Health - Category: Environmental Health Authors: Tags: Article Source Type: research
This study showed the natural evolution of indigenous fermented meat starter cultures and confirmed a higher suitability of horse and beef meat for nitrate/nitrite free salami production due to their hygienic quality at 30 d. PMID: 31948626 [PubMed - in process]
Source: Food Microbiology - Category: Food Science Authors: Tags: Food Microbiol Source Type: research
Acinetobacter baumannii (A. baumannii) is an important opportunistic pathogen causing serious nosocomial infections, which is considered as the most threatening Gram-negative bacteria (GNB). Outer membrane protei...
Source: Journal of Biomedical Science - Category: Biomedical Science Authors: Tags: Review Source Type: research
Publication date: Available online 15 January 2020Source: Microbiological ResearchAuthor(s): Ashley N. Williams, John StavrinidesAbstractMulti-drug resistant Acinetobacter baumannii and Pseudomonas aeruginosa continue to pose a serious health threat worldwide. Two Pantoea agglomerans strains, 3581 and SN01080, produce an antibiotic effective against these pathogens. To identify the antibiotic biosynthetic gene clusters, independent genetic screens were conducted for each strain using a mini-Tn5 transposon, which resulted in the identification of the same conserved eight-gene cluster. We have named this antibiotic Pantoea N...
Source: Microbiological Research - Category: Infectious Diseases Source Type: research
Authors: Escolà-Vergé L, Los-Arcos I, Almirante B Abstract One of the current priorities of the World Health Organization is multidrug-resistant bacteria, because they are a global problem due to their rapid spread and the difficulty of their treatment. In addition, they are associated with high morbidity, mortality and high economic costs. There are multidrug-resistant bacteria, both Gram-positive and Gram-negative, including Pseudomonas aeruginosa and Acinetobacter baumannii resistant to carbapenems, enterobacteria producing carbapenemases, Staphylococcus aureus resistant to methicillin and/or with ...
Source: Medicina Clinica - Category: General Medicine Tags: Med Clin (Barc) Source Type: research
Acinetobacter baumannii is a gram-negative aerobic bacillus that is commonly causes of hospital-acquired infections. Community-acquired pneumonia caused by Acinetobacter baumannii (CAP-Ab) is rare but fatal if di...
Source: BMC Infectious Diseases - Category: Infectious Diseases Authors: Tags: Case report Source Type: research
Acinetobacter baumannii is a Gram-negative opportunistic pathogen with a notorious reputation of being resistant to antimicrobial agents. The capability of A. baumannii to persist and disseminate between healthca...
Source: Annals of Clinical Microbiology and Antimicrobials - Category: Microbiology Authors: Tags: Research Source Type: research
Bacteria often exist in polymicrobial communities where they compete for limited resources. Intrinsic to this competition is the ability of some species to inhibit or kill their competitors. This phenomenon is pervasive throughout the human body where commensal bacteria block the colonization of incoming microorganisms. In this regard, molecular epidemiological and microbiota-based studies suggest that species-specific interactions play a critical role in the prevention of nasal colonization of the opportunistic pathogen Staphylococcus aureus. Despite this, S. aureus exists as part of the microbiota of ∼25% of the popu...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
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