Oral administration of transgenic biosafe microorganism containing antimicrobial peptide enhances the survival of tilapia fry infected bacterial pathogen

Publication date: December 2019Source: Fish &Shellfish Immunology, Volume 95Author(s): Bing-Chang Lee, Chun-Wei Hung, Cheng-Yung Lin, Chen-Han Shih, Huai-Jen TsaiAbstractTo develop an alternative to conventional antibiotics used in the aquaculture and livestock industries, we employed Bacillus subtilis, considered a biosafe microorganism, to express the degradable antimicrobial peptide lactoferricin. An expression plasmid pP43-6LFBII-GFP, in which reporter GFP cDNA was fused downstream of lactoferricin cDNA driven by an endogenous constitutive P43 promoter was electroporated into B. subtilis, followed by regeneration and cultivation. The putative colonies harboring plasmids were primarily screened by PCR-amplification of lactoferricin cDNA. Four transformants which were stable inheritance of plasmid containing lactoferricin cDNA included strains T1, T4, T7 and T13. Based on Western blot and Southern blot analyses, we found that transgenic strains T1 and T13 not only highly expressed exogenous recombinant lactoferricin, but also exhibited more stable inheritance of plasmids with 931 and 647 copies per cell, respectively. In the antibacterial in vitro experiment, the bactericidal activity of each microliter of cell lysate from transgenic strains T1 and T13 (5 × 108 CFU) for Escherichia coli was equivalent to 56 and 53 ng of Ampicillin dosage, respectively, while for Staphylococcus epidermidis, the equivalency T1 and T13 was 154 and 130 ng of Ampicillin dosag...
Source: Fish and Shellfish Immunology - Category: Biology Source Type: research

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Publication date: Available online 26 January 2020Source: Food ControlAuthor(s): Hanxu Pan, Kai Dong, Lei Rao, Liang Zhao, Xiaomeng Wu, Yongtao Wang, Xiaojun LiaoAbstractEscherichia coli O157:H7, the causative agent of haemorrhagic colitis and haemolytic uremic syndrome in humans, has been implicated in large food-borne outbreaks all over the world. When confronted with harsh environmental stresses, it can enter into a viable but nonculturable (VBNC) state, which poses a great risk to food safety and public health since conventional methods are invalid to detect VBNC cells. Herein, a system for detecting VBNC state Escheri...
Source: Food Control - Category: Food Science Source Type: research
Publication date: Available online 23 January 2020Source: Pesticide Biochemistry and PhysiologyAuthor(s): Xiao-Qing Zhang, Qi Yan, Lu-Lu Li, Ji-Wei Xu, Dingze Mang, Xue-Liang Wang, Hong-Huat Hoh, Jia Ye, Qian Ju, Yu Ma, Meng Liang, Yun-Ying Zhang, Xiu-Yun Zhu, Fan Zhang, Shuang-Lin Dong, Ya-Nan Zhang, Long-Wa ZhangAbstractAthetis lepigone (Alep) is a polyphagous pest native to Europe and Asia that has experienced major outbreaks in the summer maize area of China since 2011 and has shown evidence of resistance to some insecticides. Insect olfaction is crucial for recognition of sex pheromones, host plant volatiles and even ...
Source: Pesticide Biochemistry and Physiology - Category: Biochemistry Source Type: research
Human noroviruses (HuNoVs) cause sporadic and epidemic outbreaks of gastroenteritis in all age groups worldwide. We previously reported that stem cell-derived human intestinal enteroid (HIE) cultures support replication of multiple HuNoV strains and that some strains (e.g., GII.3) replicate only in the presence of bile. Heat- and trypsin-treatment of bile...
Source: Proceedings of the National Academy of Sciences - Category: Science Authors: Tags: Biological Sciences Source Type: research
This study sheds light on the virulence and AMR potential of E. coli ST302 strains and informs AMR genomic surveillance.
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
The objective of this study was to study the inactivation kinetics of Escherichia coli O157:H7 and Salmonella Typhimurium under microwave pasteurization at temperatures between 80 and 90 °C, i.e., at conditions that are employed in conventional pasteurization. Inoculated juices were treated at different power levels (600 W, 720 W) and treatment times (5s, 10s, 15s, 20s, 25s). Time-temperature profiles were obtained by fiber-optic sensors in contact with the samples allowing continuous data collection. The log-logistic and Arrhenius equations were used to account for the influence of the temperature histo...
Source: Food Microbiology - Category: Food Science Authors: Tags: Food Microbiol Source Type: research
Abstract Wheat flour has recently been recognised as an exposure vehicle for the foodborne pathogen Shiga toxin-producing Escherichia coli (STEC). Wheat flour milled on two sequential production days in October 2016, and implicated in a Canada wide outbreak of STEC O121:H19, was analysed for the presence of STEC in November 2018. Stored in sealed containers at ambient temperature, the water activity of individual flour samples was below 0.5 at 6 months post-milling and remained static or decreased slightly in individual samples during 18 months of additional storage. STEC O121 was isolated, with the same geno...
Source: Food Microbiology - Category: Food Science Authors: Tags: Food Microbiol Source Type: research
The contaminated romaine lettuce, which was harvested Salinas, California, sickened 167 people in 27 states.
Source: Health News: CBSNews.com - Category: Consumer Health News Source Type: news
The US Centers for Disease Control and the US Food and Drug Administration have declared that the recent nationwide E. Coli outbreak, which contaminated romaine lettuce, ended as of Wednesday. Authorities traced the outbreak back to the Salinas Valley growing region in California. The FDA has lifted a consumer advisory to avoid romaine lettuce from Salinas “as the growing season for this region is over and there is no longer a need for consumers to avoid it.” Health officials had previously advised consumers, retailers and restaurants to throw out any romaine lettuce grown in the Salinas, Calif. region. People...
Source: TIME: Health - Category: Consumer Health News Authors: Tags: Uncategorized onetime public health Source Type: news
But as of Wednesday, the outbreak of E. coli illnesses linked to the lettuce "appears to be over," the CDC said in a statement.
Source: WebMD Health - Category: Consumer Health News Source Type: news
(CNN) — Caesar salad lovers rejoice — your crispy romaine lettuce leaves are OK to eat now. The nationwide, monthslong E. coli outbreak linked to romaine lettuce ended Wednesday, according to the US Centers for Disease Control and Prevention and the US Food and Drug Administration. The romaine, which came from Salinas, California, infected a total of 167 people in 27 states. This included 85 hospitalizations, including 15 patients who developed a type of kidney failure — hemolytic uremic syndrome — known to be associated with this particular type of bacteria, E. coli O157:H7. The toxin produced by t...
Source: WBZ-TV - Breaking News, Weather and Sports for Boston, Worcester and New Hampshire - Category: Consumer Health News Authors: Tags: Health News Syndicated CBSN Boston CNN Romaine Lettuce Source Type: news
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