pH-induced variations in the TK1 gene model
Publication date: Available online 28 November 2019Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Julien Massonneau, Chloë Lacombe-Burgoyne, Guylain BoissonneaultAbstractA physiological decrease in extracellular pH (pHe) alters the efficiency of DNA repair and increases formation of DNA double-strand breaks (DSBs). Whether this could translate into genetic instability and variations, was investigated using the TK6 cell model, in which positive selection of the TK1 gene loss-of-function mutations can be achieved from resistance to trifluorothymidine. Cell exposure to suboptimal pH (down to 6.9) for 3 weeks resulted in the 100% frequency of a stronger frameshift mutation that has spread to both TK1 alleles, whereas weaker frameshift mutations within the 3’exon were eliminated during the selection. Suboptimal pHe values were also found to alter the proportion of the TK1 splicing variant expressed as percent spliced in index values and promote selection of truncated exons as well as intron retention. Although recovery at pH 7.4 did not reverse the selected frameshift mutation, reversal of splice variants and exon truncation towards control values were observed. Hence, suboptimal pHe can induce a combination of mutational events and splicing alterations within the same gene in the resistant clones. This model of positive selection for loss-of-function clearly demonstrates that suboptimal pHe may confer a similar growth advantage whe...
Publication date: Available online 4 April 2020Source: International Journal of Surgery Case ReportsAuthor(s): Bianca Kwan, Jane E. Theodore, Jason Wong
I don't want to get too into it, I wouldn't call this like a "full" wamc or whatever but i have no clue on how to make a list with my stats about the author: 3.41 sgpa, cgpa 3.39. i would like to do gpa repair but due to many reasons its just not financially feasible. grad from jhu so hopefully explains the gpa a little bit?? but still obviously bad, and im hoping my mcat trend sells this idea that im better than i used to be. mcats 502->511->526. nontangibles: ok volunteering (like 300... low gpa, high retake, where to apply?
Publication date: Available online 3 April 2020Source: Orthopaedics &Traumatology: Surgery &ResearchAuthor(s): Gilles Guérin, Nicolas Pujol
Publication date: Available online 3 April 2020Source: The Annals of Thoracic SurgeryAuthor(s): Mehmet Alagoz, Halil Sencer Akdeniz, Ahmet Dolapoglu
Publication date: Available online 3 April 2020Source: The Annals of Thoracic SurgeryAuthor(s): Daniel L. Miller
Abstract Repair of injured tissues requires angiogenesis, the growth of new blood vessels from pre-existing ones. Cutaneous wound healing is a complex and dynamic process by which skin tissue repairs itself after injury; however, how endothelial cells and pericytes form new blood vessels during cutaneous wound angiogenesis remains unclear. We recently developed a fluorescence-based live imaging system to analyze cutaneous wound angiogenesis in adult zebrafish. Employing this system, we found that endothelial cells and pericytes remain in a quiescent state in normal skin tissue, whereas cutaneous injury immediately...
CONCLUSIONS: Despite more comorbidities in the over 65s, this study shows that there is no significant difference in complication rates between laparoscopic and open inguinal hernia repair irrespective of age category. Selection bias for the type of repair and the potential for an alpha error mean larger studies are required to show equivalence. PMID: 32243111 [PubMed - as supplied by publisher]
CONCLUSION: Delayed repair, up to 2 months later, for uncomplicated infant hernia carries a small risk of incarceration but does not increase the rate of strangulation or other complications. PMID: 32243110 [PubMed - as supplied by publisher]
CONCLUSION: Gallbladder perforations are common during laparoscopic cholecystectomy due to traction with forceps or inflammation. Careful inspection for spillage stone should be done. KEY WORDS: Laparoscopic cholecystectomy, Retained Stones, hernia, Trocar Site. PMID: 32242545 [PubMed - in process]
Conclusion. The ankle ligament reconstruction with additional polyester tape augmentation is an effective technique in treating chronic ankle instability with a satisfactory surgical outcome. PMID: 32242523 [PubMed - in process]