Nature and consequences of interactions between Salmonella enterica serovar Dublin and host cells in cattle

AbstractSalmonella enterica is a veterinary and zoonotic pathogen of global importance. While murine and cell-based models of infection have provided considerable knowledge about the molecular basis of virulence ofSalmonella, relatively little is known about salmonellosis in naturally-affected large animal hosts such as cattle, which are a reservoir of human salmonellosis. As in humans,Salmonella causes bovine disease ranging from self-limiting enteritis to systemic typhoid-like disease and exerts significant economic and welfare costs. Understanding the nature and consequences ofSalmonella interactions with bovine cells will inform the design of effective vaccines and interventions to control animal and zoonotic infections. In calves challenged orally withS. Dublin expressing green fluorescent protein (GFP) we observed that the bacteria were predominantly extracellular in the distal ileal mucosa and within gut-associated lymph nodes 48  h post-infection. Intracellular bacteria, identified by flow cytometry using the GFP signal, were predominantly within MHCII+ macrophage-like cells. In contrast to observations from murine models, theseS. Dublin-infected cells had elevated levels of MHCII and CD40 compared to both uninfected cells from the same tissue and cells from the cognate tissue of uninfected animals. Moreover, no gross changes of the architecture of infected lymph nodes were observed as was described previously in a mouse model. In order to further investigateSalm...
Source: Veterinary Research - Category: Veterinary Research Source Type: research

Related Links:

CONCLUSION We identified a population of HCV-specific CD4+ T cells with a follicular T helper cell signature that is maintained after therapy-induced elimination of persistent infection and may constitute an important target population for vaccination efforts to prevent reinfection and immunotherapeutic approaches for persistent viral infections.FUNDING Deutsche Forschungsgemeinschaft (DFG, German Research Foundation), the National Institute of Allergy and Infectious Diseases (NIAID), the European Union, the Berta-Ottenstein-Programme for Advanced Clinician Scientists, and the ANRS.
Source: Journal of Clinical Investigation - Category: Biomedical Science Authors: Source Type: research
[Nation] Tax payers have lost Sh92 million after a new foot-and-mouth disease vaccine that had been manufactured by the veterinary agency expired following a tug-of-war between senior government officials and the corporation over a benchmarking trip.
Source: AllAfrica News: Health and Medicine - Category: African Health Source Type: news
(University of Michigan) Fully vaccinating children reduces the risk of hospitalization associated with influenza by 54%, according to a study by researchers at the University of Michigan, the Clalit Research Institute, and Ben-Gurion University in Israel.
Source: EurekAlert! - Medicine and Health - Category: International Medicine & Public Health Source Type: news
Publication date: Available online 21 January 2020Source: The LancetAuthor(s): Maria Brotons, Laia Bruni
Source: The Lancet - Category: General Medicine Source Type: research
Publication date: Available online 21 January 2020Source: The LancetAuthor(s): Mélanie Drolet, Marc Brisson
Source: The Lancet - Category: General Medicine Source Type: research
Publication date: Available online 21 January 2020Source: The LancetAuthor(s): Peter Sasieni, Jack Cuzick
Source: The Lancet - Category: General Medicine Source Type: research
In this study, we challenged three independent chick populations (Beijing You, Dwarf and Rhode Island Red) with SP at 4 days of age, and rectal temperature was measured before and after the SP attack from 2 to 7 days of age. Host defense to SP was evaluated by survival and spleen SP carrier status. The results showed that chicks with higher EBT before SP infection tend to have higher resistance to later SP attack in two populations (Dwarf and Beijing You). The association between EBT before SP attack and SP resistance was non-significant in Rohde Island Red population (P = 0.06), but the trend was consistent with the other...
Source: Frontiers in Genetics - Category: Genetics & Stem Cells Source Type: research
Publication date: Available online 20 January 2020Source: Ticks and Tick-borne DiseasesAuthor(s): Luís Fernando Parizi, Carolina Konrdörfer Rangel, Gabriela Alves Sabadin, Bianca Fagundes Saggin, Irene Kiio, Marina Amaral Xavier, Renata da Silva Matos, Maria Izabel Camargo-Mathias, Adriana Seixas, Satoru Konnai, Kazuhiko Ohashi, Naftaly Wang'ombe Githaka, Itabajara da Silva VazAbstractRhipicephalus appendiculatus, the brown ear tick, is an important disease vector of livestock in eastern, central and southern Africa. Rhipicephalus appendiculatus acaricide resistance requires the search for alternative methods f...
Source: Ticks and Tick borne Diseases - Category: Zoology Source Type: research
Publication date: Available online 16 July 2019Source: Food Research InternationalAuthor(s): Ines Staes, Ioannis Passaris, Alexander Cambré, Abram AertsenAbstractSalmonella enterica comprises many pathogenic serovars that are able to colonize a variety of animal hosts and therefore constitute an important source of zoonotic food-borne illness. Their pathogenicity can range from gastroenteritis to typhoid fever, and depends on a series of virulence factors that are regularly located on laterally acquired genetic elements. The regulation of these virulence factors often also includes their differential expression with...
Source: Food Research International - Category: Food Science Source Type: research
This report describes the dynamics of FQ-associated mutations in the highly resistant in FQ mutants in S. Enteritidis. In addition, we characterized a deletion in the ramRA integenic region, demonstrating that this frequent mutation in the highly resistant FQ mutants provide resistance or reduce susceptibility to multiple families of antibiotics. Introduction Non-typhoidal Salmonella (NTS) is a major zoonotic pathogen worldwide (Bangtrakulnonth et al., 2004; Scallan et al., 2011). Infections caused by this pathogen have been mainly associated with gastroenteritis, an acute self-limiting intestinal infection. Howeve...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
More News: Salmonella | Typhoid | Typhoid Vaccine | Vaccines | Veterinary Research | Veterinary Vaccinations | Zoonoses