Deep-supercooling for extended preservation of adipose-derived stem cells.

Deep-supercooling for extended preservation of adipose-derived stem cells. Cryobiology. 2019 Nov 18;: Authors: Huang H, Bedon CR, Yarmush ML, Usta OB Abstract Cell preservation is an enabling technology for widespread distribution and applications of mammalian cells. Traditional cryopreservation via slow-freezing or vitrification provides long-term storage but requires cytotoxic cryoprotectants (CPA) and tedious CPA loading, cooling, and recovering procedures. Hypothermic storage around 0-4 °C is an alternative method but only works for a short period due to its high storage temperature. Here, we report on the deep-supercooling (DSC) preservation of human adipose-derived stem cells at deep subzero temperatures without freezing for extended storage. Enabled by surface sealing with an immiscible oil phase, cell suspension can be preserved in a liquid state at -13 °C and -16 °C for 7 days with high cell viability; and retention of stemness, attachment, and multilineage differentiation capacities. These results demonstrate that DSC is an improved short-term preservation approach to provide off-the-shelf cell sources for booming cell-based medicine and bioengineering. PMID: 31751557 [PubMed - as supplied by publisher]
Source: Cryobiology - Category: Biology Authors: Tags: Cryobiology Source Type: research